Abstract
The response to chronic hypertonic stress has been studied in human endothelial cells derived from saphenous veins. In complete growth medium the full recovery of cell volume requires several hours and is neither associated with an increase in cell K+ nor hindered by bumetanide but depends on an increased intracellular pool of amino acids. The highest increase is exhibited by neutral amino acid substrates of transport system A, such as glutamine and proline, and by the anionic amino acid glutamate. Transport system A is markedly stimulated on hypertonic stress, with an increase in activity roughly proportional to the extent and the duration of the osmotic shrinkage. Cycloheximide prevents the increase in transport activity of system A and the recovery of cell volume. It is concluded that human endothelial cells counteract hypertonic stress through the stimulation of transport system A and the consequent expansion of the intracellular amino acid pool.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 276 |
| Issue number | 4 45-4 |
| Publication status | Published - 1999 |
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Keywords
- Amino acid transport
- Bumetanide
- Glutamine
- Regulatory volume increase
- System A
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
- Physiology (medical)
Cite this
Amino acids are compatible osmolytes for volume recovery after hypertonic shrinkage in vascular endothelial cells. / Dall'Asta, Valeria; Bussolati, Ovidio; Sala, Roberto; Parolari, Alessandro; Alamanni, Francesco; Biglioli, Paolo; Gazzola, Gian C.
In: American Journal of Physiology - Cell Physiology, Vol. 276, No. 4 45-4, 1999.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amino acids are compatible osmolytes for volume recovery after hypertonic shrinkage in vascular endothelial cells
AU - Dall'Asta, Valeria
AU - Bussolati, Ovidio
AU - Sala, Roberto
AU - Parolari, Alessandro
AU - Alamanni, Francesco
AU - Biglioli, Paolo
AU - Gazzola, Gian C.
PY - 1999
Y1 - 1999
N2 - The response to chronic hypertonic stress has been studied in human endothelial cells derived from saphenous veins. In complete growth medium the full recovery of cell volume requires several hours and is neither associated with an increase in cell K+ nor hindered by bumetanide but depends on an increased intracellular pool of amino acids. The highest increase is exhibited by neutral amino acid substrates of transport system A, such as glutamine and proline, and by the anionic amino acid glutamate. Transport system A is markedly stimulated on hypertonic stress, with an increase in activity roughly proportional to the extent and the duration of the osmotic shrinkage. Cycloheximide prevents the increase in transport activity of system A and the recovery of cell volume. It is concluded that human endothelial cells counteract hypertonic stress through the stimulation of transport system A and the consequent expansion of the intracellular amino acid pool.
AB - The response to chronic hypertonic stress has been studied in human endothelial cells derived from saphenous veins. In complete growth medium the full recovery of cell volume requires several hours and is neither associated with an increase in cell K+ nor hindered by bumetanide but depends on an increased intracellular pool of amino acids. The highest increase is exhibited by neutral amino acid substrates of transport system A, such as glutamine and proline, and by the anionic amino acid glutamate. Transport system A is markedly stimulated on hypertonic stress, with an increase in activity roughly proportional to the extent and the duration of the osmotic shrinkage. Cycloheximide prevents the increase in transport activity of system A and the recovery of cell volume. It is concluded that human endothelial cells counteract hypertonic stress through the stimulation of transport system A and the consequent expansion of the intracellular amino acid pool.
KW - Amino acid transport
KW - Bumetanide
KW - Glutamine
KW - Regulatory volume increase
KW - System A
UR - http://www.scopus.com/inward/record.url?scp=0032929352&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032929352&partnerID=8YFLogxK
M3 - Article
C2 - 10199817
AN - SCOPUS:0032929352
VL - 276
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 4 45-4
ER -