Amniotic membrane diffusion chambers: a new possibility for pancreatic islet transplantation.

A. Maldonato, A. Lucisano, E. Maniccia, G. Luciani, R. Agnes, S. Magalini, A. Cama, F. Marani, F. Barbetti, M. Fattibene

Research output: Contribution to journalArticlepeer-review

Abstract

In order to avoid the host foreign-body reaction which is probably responsible for the long-term failure of the artificial membrane diffusion chambers currently used for protecting the implanted islet tissue from rejection, we tested the human amniotic membrane as a possible new tool for enclosing transplanted islets of Langerhans. Bag-shaped chambers were made with human amniotic membranes and the diffusion of 125 I-Insulin out and of D-glucose into the chambers was measured. The diffusion patterns of insulin and glucose were shown to be better than those described for the best artificial membranes, the 50% diffusion being reached at min 42 for 125 I-Insulin and at min 11 for D-glucose. Moreover, it was shown that no lymphocytes diffused into the amniotic membrane chambers. These results, together with the already known characteristics of the human amniotic membrane, induce us to propose it as a suitable material for further experimental work in islet tissue transplantation.

Original languageEnglish
Pages (from-to)640-644
Number of pages5
JournalLife Support Systems
Volume3 Suppl 1
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Medicine(all)

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