Amphetamine exposure imbalanced antioxidant activity in the bivalve Dreissena polymorpha causing oxidative and genetic damage

Marco Parolini, Stefano Magni, Sara Castiglioni, Andrea Binelli

Research output: Contribution to journalArticle

Abstract

Illicit drugs have been recognized as emerging aquatic pollutants due to their presence in aquatic ecosystems up to μg/L level. Among these, the synthetic psycho-stimulant drug amphetamine (AMPH) is commonly found in both surface and wastewaters worldwide. Even though the environmental occurrence of AMPH is well-known, the information on its toxicity towards non-target freshwater organisms is completely lacking. This study investigated the imbalance of the oxidative status and both oxidative and genetic damage induced by a 14-day exposure to two concentrations (500 ng/L and 5000 ng/L) of AMPH on the freshwater bivalve Dreissena polymorpha by the application of a biomarker suite. We investigated the activity of antioxidant enzymes (SOD, CAT and GPx), the phase II detoxifying enzyme GST, the lipid peroxidation level (LPO) and protein carbonyl content (PCC), as well as primary (Single Cell Gel Electrophoresis assay) and fixed (DNA diffusion assay and Micronucleus test) genetic damage. Our results showed that a current realistic AMPH concentration (500 ng/L) did neither cause notable imbalances in enzymatic activities, nor oxidative and genetic damage to cellular macromolecules. In contrast, the bell-shaped trend of antioxidants showed at the highest tested concentration (5000 ng/L) suggested an overproduction of reactive oxygen species, leading to oxidative damage, as confirmed by the significant increase of protein carbonylation and DNA fragmentation.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalChemosphere
Volume144
DOIs
Publication statusPublished - Feb 1 2016

Fingerprint

Dreissena
Bivalvia
Amphetamine
Antioxidants
antioxidant
bivalve
Assays
DNA
Enzymes
Proteins
Carbonylation
Aquatic ecosystems
damage
Biomarkers
Electrophoresis
Fresh Water
Macromolecules
Lipids
Toxicity
Wastewater

Keywords

  • Amphetamine
  • Dreissena polymorpha
  • Illicit drugs
  • Oxidative and genetic biomarkers

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Amphetamine exposure imbalanced antioxidant activity in the bivalve Dreissena polymorpha causing oxidative and genetic damage. / Parolini, Marco; Magni, Stefano; Castiglioni, Sara; Binelli, Andrea.

In: Chemosphere, Vol. 144, 01.02.2016, p. 207-213.

Research output: Contribution to journalArticle

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