Amphibian peptides prevent endotoxemia and bacterial translocation in bile duct-ligated rats

Andrea Giacometti, Oscar Cirioni, Roberto Ghiselli, Federico Mocchegiani, Carmela Silvestri, Fiorenza Orlando, Wojciech Kamysz, Alberto Licci, Elzbieta Kamysz, Jerzy Łukasiak, Vittorio Saba, Giorgio Scalise

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To investigate the efficacy of amphibian antimicrobial peptides in preventing bacterial translocation and neutralizing endotoxins in bile duct-ligated rats. DESIGN: Prospective, randomized, controlled animal study. SETTING: Research laboratory in a university hospital. SUBJECTS: Adult male Wistar rats. INTERVENTIONS: Adult male Wistar rats underwent sham operation or bile duct ligation (BDL). Eight groups were studied: sham operation with saline treatment, sham operation with 120 mg/kg tazobactam-piperacillin, sham operation with 2 mg/kg uperin 3.6, sham operation with 2 mg/kg magainin2, BDL with saline treatment, BDL with 120 mg/kg tazobactam-piperacillin, BDL with 2 mg/kg uperin 3.6, and BDL with 2 mg/kg magainin2. MEASUREMENTS AND MAIN RESULTS: Main outcome measures were: endotoxin and tumor necrosis factor-α concentrations in plasma and evidence of bacterial translocation in blood, peritoneum, liver, and mesenteric lymph nodes. Endotoxin and tumor necrosis factor-α plasma levels were significantly higher in BDL rats compared with sham-operated animals. All amphibian peptides achieved a significant reduction of plasma endotoxin and tumor necrosis factor-α concentration when compared with saline- and tazobactam-piperacillin-treated groups. On the other hand, both tazobactam-piperacillin and peptides significantly reduced bacterial growth compared with the control. Tazobactam-piperacillin and magainin2 exerted the maximal inhibition of bacterial growth. CONCLUSION: In conclusion, because of their multifunctional properties, amphibian peptides could be interesting compounds to inhibit bacterial translocation and endotoxin release in obstructive jaundice.

Original languageEnglish
Pages (from-to)2415-2420
Number of pages6
JournalCritical Care Medicine
Volume34
Issue number9
DOIs
Publication statusPublished - Sep 2006

Keywords

  • Amphibian peptides
  • Animal model
  • Antimicrobial peptides
  • Bacterial translocation
  • Obstructive jaundice

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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