Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing

Pierre Wenger, Maxime de Zuanni, Philippe Javet, Cecilia Gelfi, Pier Giorgio Righetti

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Amphoteric, isoelectric agarose membranes, as devised by Martin and Hampson [Martin, A.J.P. and Hampson, F. (1987) J. Chromatogr. 159, 101-110], are found unsuitable for blocking electroendosmosis in multi-compartment electrolysers during preparative isoelectric focusing, due to the poor and highly unpredictable incorporation of carboxyls and amino groups on the polysaccharide moiety. New, polyacrylamide-based membranes are describes, containing as buffers and titrants the Immobiline chemicals used to produce immobilized pH gradients. These new membranes are supported on both faces by a non-woven polypropylene cloth, a material exhibiting minimal adsorption properties for proteins. Due to the extensively developed Immobiline technology, membranes with highly predictable isoelectric points, well-defined buffering capacity and conductivity can be synthesized at any pH value along the pH 3-10 scale. They are effective in blocking electroendosmosis even when the ΔpH either side of the membrane is as high as 1.5 pH unit.

Original languageEnglish
Pages (from-to)29-43
Number of pages15
JournalJournal of Biochemical and Biophysical Methods
Volume14
Issue number1
DOIs
Publication statusPublished - 1987

Fingerprint

Isoelectric Focusing
Electroosmosis
Membranes
Membrane technology
Polypropylenes
Proton-Motive Force
Sepharose
Polysaccharides
Isoelectric Point
Buffers
Adsorption
Immobiline
Technology
Proteins

Keywords

  • Amphoteric membrane
  • Immobilized pH gradient
  • Multicompartment electrolyzer
  • Preparative isoelectric focusing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing. / Wenger, Pierre; de Zuanni, Maxime; Javet, Philippe; Gelfi, Cecilia; Righetti, Pier Giorgio.

In: Journal of Biochemical and Biophysical Methods, Vol. 14, No. 1, 1987, p. 29-43.

Research output: Contribution to journalArticle

Wenger, Pierre ; de Zuanni, Maxime ; Javet, Philippe ; Gelfi, Cecilia ; Righetti, Pier Giorgio. / Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing. In: Journal of Biochemical and Biophysical Methods. 1987 ; Vol. 14, No. 1. pp. 29-43.
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