Amyloid β peptide-induced inhibition of endothelial nitric oxide production involves oxidative stress-mediated constitutive eNOS/HSP90 interaction and disruption of agonist-mediated Akt activation

Folami Lamoke, Valeria Mazzone, Tiziana Persichini, Annamaria Maraschi, Brennan M. Harris, Richard C. Venema, Marco Colasanti, Micaela Gliozzi, Carolina Muscoli, Manuela Bartoli, Vincenzo Mollace

Research output: Contribution to journalArticle

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Abstract

Background: Amyloid β (Aβ)-induced vascular dysfunction significantly contributes to the pathogenesis of Alzheimer's disease (AD). Aβ is known to impair endothelial nitric oxide synthase (eNOS) activity, thus inhibiting endothelial nitric oxide production (NO). Method: In this study, we investigated Aβ-effects on heat shock protein 90 (HSP90) interaction with eNOS and Akt in cultured vascular endothelial cells and also explored the role of oxidative stress in this process. Results: Treatments of endothelial cells (EC) with Aβ promoted the constitutive association of HSP90 with eNOS but abrogated agonist (vascular endothelial growth factor (VEGF))-mediated HSP90 interaction with Akt. This effect resulted in blockade of agonist-mediated phosphorylation of Akt and eNOS at serine 1179. Furthermore, Aβ stimulated the production of reactive oxygen species in endothelial cells and concomitant treatments of the cells with the antioxidant N-acetyl-cysteine (NAC) prevented Aβ effects in promoting HSP90/eNOS interaction and rescued agonist-mediated Akt and eNOS phosphorylation. Conclusions: The obtained data support the hypothesis that oxidative damage caused by Aβ results in altered interaction of HSP90 with Akt and eNOS, therefore promoting vascular dysfunction. This mechanism, by contributing to Aβ-mediated blockade of nitric oxide production, may significantly contribute to the cognitive impairment seen in AD patients.

Original languageEnglish
Article number84
JournalJournal of Neuroinflammation
Volume12
Issue number1
DOIs
Publication statusPublished - May 3 2015

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HSP90 Heat-Shock Proteins
Nitric Oxide Synthase Type III
Amyloid
Nitric Oxide
Oxidative Stress
Peptides
Endothelial Cells
Blood Vessels
Alzheimer Disease
Phosphorylation
Inhibition (Psychology)
Serine
Vascular Endothelial Growth Factor A
Cysteine
Reactive Oxygen Species
Antioxidants
Therapeutics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience
  • Neurology
  • Immunology

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Amyloid β peptide-induced inhibition of endothelial nitric oxide production involves oxidative stress-mediated constitutive eNOS/HSP90 interaction and disruption of agonist-mediated Akt activation. / Lamoke, Folami; Mazzone, Valeria; Persichini, Tiziana; Maraschi, Annamaria; Harris, Brennan M.; Venema, Richard C.; Colasanti, Marco; Gliozzi, Micaela; Muscoli, Carolina; Bartoli, Manuela; Mollace, Vincenzo.

In: Journal of Neuroinflammation, Vol. 12, No. 1, 84, 03.05.2015.

Research output: Contribution to journalArticle

Lamoke, Folami ; Mazzone, Valeria ; Persichini, Tiziana ; Maraschi, Annamaria ; Harris, Brennan M. ; Venema, Richard C. ; Colasanti, Marco ; Gliozzi, Micaela ; Muscoli, Carolina ; Bartoli, Manuela ; Mollace, Vincenzo. / Amyloid β peptide-induced inhibition of endothelial nitric oxide production involves oxidative stress-mediated constitutive eNOS/HSP90 interaction and disruption of agonist-mediated Akt activation. In: Journal of Neuroinflammation. 2015 ; Vol. 12, No. 1.
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