TY - JOUR
T1 - Amyloid fibrils containing fragmented ATTR may be the standard fibril composition in ATTR amyloidosis
AU - Ihse, Elisabet
AU - Rapezzi, Claudio
AU - Merlini, Giampaolo
AU - Benson, Merrill D.
AU - Ando, Yukio
AU - Suhr, Ole B.
AU - Ikeda, Shu Ichi
AU - Lavatelli, Francesca
AU - Obici, Laura
AU - Quarta, Candida C.
AU - Leone, Ornella
AU - Jono, Hirofumi
AU - Ueda, Mitsuharu
AU - Lorenzini, Massimiliano
AU - Liepnieks, Juris
AU - Ohshima, Toshinori
AU - Tasaki, Masayoshi
AU - Yamashita, Taro
AU - Westermark, Per
PY - 2013/9
Y1 - 2013/9
N2 - The clinical phenotype of familial ATTR amyloidosis depends to some extent on the particular mutation, but differences exist also within mutations. We have previously described that two types of amyloid fibril compositions exist among Swedish ATTRV30M amyloidosis patients, one consisting of a mixture of intact and fragmented ATTR (type A) and one consisting of mainly intact ATTR (type B). The fibril types are correlated to phenotypic differences. Patients with ATTR fragments have a late onset and develop cardiomyopathy, while patients without fragments have an early onset and less myocardial involvement. The present study aimed to determine whether this correlation between fibril type and phenotype is valid for familial ATTR amyloidosis in general. Cardiac or adipose tissues from 63 patients carrying 29 different TTR non-V30M mutations as well as 13 Japanese ATTRV30M patients were examined. Fibril type was determined by western blotting and compared to the patients' age of onset and degree of cardiomyopathy. All ATTR non-V30M patients had a fibril composition with ATTR fragments, except two ATTRY114C patients. No clear conclusions could be drawn about a phenotype to fibril type correlation among ATTR non-V30M patients. In contrast, Japanese ATTRV30M patients showed a similar correlation as previously described for Swedish ATTRV30M patients. This study shows that a fibril composition with fragmented ATTR is very common in ATTR amyloidosis, and suggests that fibrils composed of only full-length ATTR is an exception found only in a subset of patients.
AB - The clinical phenotype of familial ATTR amyloidosis depends to some extent on the particular mutation, but differences exist also within mutations. We have previously described that two types of amyloid fibril compositions exist among Swedish ATTRV30M amyloidosis patients, one consisting of a mixture of intact and fragmented ATTR (type A) and one consisting of mainly intact ATTR (type B). The fibril types are correlated to phenotypic differences. Patients with ATTR fragments have a late onset and develop cardiomyopathy, while patients without fragments have an early onset and less myocardial involvement. The present study aimed to determine whether this correlation between fibril type and phenotype is valid for familial ATTR amyloidosis in general. Cardiac or adipose tissues from 63 patients carrying 29 different TTR non-V30M mutations as well as 13 Japanese ATTRV30M patients were examined. Fibril type was determined by western blotting and compared to the patients' age of onset and degree of cardiomyopathy. All ATTR non-V30M patients had a fibril composition with ATTR fragments, except two ATTRY114C patients. No clear conclusions could be drawn about a phenotype to fibril type correlation among ATTR non-V30M patients. In contrast, Japanese ATTRV30M patients showed a similar correlation as previously described for Swedish ATTRV30M patients. This study shows that a fibril composition with fragmented ATTR is very common in ATTR amyloidosis, and suggests that fibrils composed of only full-length ATTR is an exception found only in a subset of patients.
KW - Familial amyloidotic polyneuropathy
KW - Fibril composition
KW - Transthyretin
KW - TTR non-V30M
KW - TTRV30M
KW - TTRY114C
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U2 - 10.3109/13506129.2013.797890
DO - 10.3109/13506129.2013.797890
M3 - Article
C2 - 23713495
AN - SCOPUS:84883044150
VL - 20
SP - 142
EP - 150
JO - Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis
JF - Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis
SN - 1350-6129
IS - 3
ER -