Abstract
Original language | English |
---|---|
Pages (from-to) | 265-274 |
Number of pages | 10 |
Journal | Altex |
Volume | 37 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2020 |
Keywords
- caspase 3
- collagen type 1
- collagen type 1 alpha 1
- complementary DNA
- cytochrome c
- gelatinase B
- immunoglobulin enhancer binding protein
- interleukin 1alpha
- interleukin 1beta
- interleukin 6
- interstitial collagenase
- matrigel
- nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
- peroxide
- protein Bax
- protein bcl 2
- protein p53
- second mitochondrial activator of caspase
- stromelysin
- synaptotagmin I
- transforming growth factor beta2
- tumor necrosis factor
- ubiquitin C
- unclassified drug
- ubiquitin
- antiapoptotic activity
- apoptosis assay
- Article
- chronic stress
- gene expression
- glaucoma
- human
- human cell
- immunoreactivity
- in vitro study
- metabolic activity assay
- oxidative stress
- polyacrylamide gel electrophoresis
- protein expression
- real time polymerase chain reaction
- resazurin assay
- RNA extraction
- three dimensional cell culture
- trabecular meshwork
- Western blotting
- adaptive behavior
- cell culture technique
- cell growth
- confocal microscopy
- controlled study
- cytokine production
- down regulation
- flow cytometry
- genetic transfection
- signal transduction
- upregulation
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An advanced in vitro model to assess glaucoma onset. / Sacc, S.C.; Tirendi, S.; Scarji, S.; Fassalacqua, M.; Oddone, F.; Traverso, C.K.; Vernazza, S.; Bassi, A.M.
In: Altex, Vol. 37, No. 2, 2020, p. 265-274.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - An advanced in vitro model to assess glaucoma onset
AU - Sacc, S.C.
AU - Tirendi, S.
AU - Scarji, S.
AU - Fassalacqua, M.
AU - Oddone, F.
AU - Traverso, C.K.
AU - Vernazza, S.
AU - Bassi, A.M.
N1 - Cited By :3 Export Date: 2 February 2021 CODEN: ALTEE Correspondence Address: Vernazza, S.; Department of Experimental Medicine, Italy Chemicals/CAS: caspase 3, 169592-56-7; cytochrome c, 9007-43-6, 9064-84-0; gelatinase B, 146480-36-6; interstitial collagenase, 9001-12-1; matrigel, 119978-18-6; peroxide, 14915-07-2; protein bcl 2, 219306-68-0; stromelysin, 79955-99-0; ubiquitin C, 151821-62-4; ubiquitin, 60267-61-0 References: Aarskog, N., Vedeler, C., Real-time quantitative polymerase chain reaction (2000) Hum Genet, 107, pp. 494-498; Acott, T. S., Kelley, M., Extracellular matrix in the trabecular meshwork (2008) Eye Res, 86, pp. 543-561. , 3; Ahluwalia, A., Allometric scaling in-vitro (2017) Sci Rep, 7, p. 42113; Benton, G., Arnaoutova, I., George, J., Maui- gel: From discovery and ECM mimicry to assays and mod- els for cancer research (2014) Adv Drug Del/v Rev, 79-80, pp. 3-18; Berger, E., Paczia, N., Millifluidic culture improves human midbrain organoid vitality and differentiation (2018) Lab Chip, 18, pp. 3172-3183. , Magliaro. C; Boardman., K. C., Aryal, A. M., Miller., W. M., Actin re-distribution in response to hydrogen peroxide in airway epithelial cells (2004) JCell .Physiol, 199 (5), pp. 7-66; Roubeix, C., Kessal, K., Effect of benzalkonium chloride on trabecular meshwork cells in a new in vitro 3D trabecular meshwork model for glaucoma (2017) Toxicol In Vitro, 41, pp. 21-29. , Bouchemi. M; Bouhenni, P.. A., Dunmire, 3., Sewell, A., Animal models of glaucoma (2012) J Biomed Biotechnol, 2012, p. 692609; Brancato, V., Gioiella, F., 3D breast cancer microtissue reveals the role of tumor microenvironment on the transport and efficacy of free-doxorubicin in vitro (2018) Acta Biomater, 75, pp. 200-212. , Imparato. 0; Burgoyne, C. F., The non-human primate experimental glaucoma model (2015) Eye Res, 141, pp. 57-73; Elmore, S., Apoptosis: Areview of programmed cell death (2007) ToxicolPathol35, 495-5 16; de-la-Torre, A., Experimental models of glaucoma: A powerful translational tool for the future development of new therapies for glaucoma in humans 1/2A review of the literature (2019) Med/dna (Ma), 55, p. 280. , Evangelho. K., Mastronardi. C. A. and; Fuchshofer, R., Modulation of extracellular matrix turnover in the trabecular meshwork (2009) Erp Eye Res, 88, pp. 683-688. , and Tamm. E. R; Giusti, S., La, Marca. M., A novel dual-flow bioreactor simulates increased fluorescein permeability in epithelial tissue barriers (2014) Biotechnol J, 9, pp. 1175-1184. , Sbrana. T; Gonzalez, J. M., Hamm-Alvarez, . S., Tan, 3. C. H., Analyzing live cellularity in the human trabecular meshwork (2013) Invest Opthalmol fir SeE, 54, pp. 1039-1047; Hughes, C. S., Postovit, L. M., Lajoie, G. A., Math- gel: A complex protein mixture required for optimal growth of cell culture (2010) Proteomics, 10, pp. 1886-1890; Ishikawa, M., Yoshitomi, T., Zorumski, C. F., Experimentally induced mammalian models of glaucoma (2015) Biomed ResInt2OlS, p. 281214. , lO.1155/2015/281214; Izzotti, A., Longobardi, S. C.., Sensitivity of ocular anterior chamber tissues to oxidative damage and its relevance to the pathogenesis of glaucoma (2009) Invest Ophthalmol V/s SeE, 50, pp. 5251-5258. , Sacc 1/2 M. et al. lO.1l67/iovs.09-387l; Dynamics of H202 availability to ARPE- 19 cultures in models of oxidative stress (2010) Free Radic Blot Med, 48, pp. 1064-1070. , Kaczara. P., Sarna. T. and Burke, 3. M; Keller, K. E., Bhattacharya, S. K., Consensus recommendations for trabecular meshwork cell isola tion, characterization and culture (2018) ELtp Eye Res, 171, pp. 164-173. , Borr 1/2s, T; Kim, H., Inhibitory effect of astaxanthin on oxidative stress-induced mitochondrial dysfunction 1/2 A mini-review (2018) Nutrients, 10, p. 1137. , Kim. S. H. and; Kim, Y. W., Park, K. H., Exogenous influences on intraocular pressure (2019) Br J Ophthalmol, 2018, p. 313381; Kohen, N. T., Little, L. E., Healy, K. E., Characterization of Matrigel interfaces during defined human embryonic stem cell culture (2009) Bzoznterphases, 4, pp. 69-79; Adcock, I. M., Towards a 2P-century roadmap for biomedical research and drug discovery: Consensus report and recommendations (2017) Drug Discov Today, 22, pp. 327-339. , Busquet. F Langley. 0. R; Li, O., Lee, C., Agrahari, V., In vivo measurement of trabecular meshwork stifibess in a corticosteroid-induced ocular hypertensive mouse model (2019) Proc NatlAcadSci USA, 116, pp. 1714-1722. , lO.l073/pnas.l814889ll6; Lv, X., Liu, S., Hu, Z.-W., Autophagy-inducing natural compounds: A treasure resource for developing therapeutics against tissue fibrosis (2017) JAsian Nat Prod Res, 19, pp. 101-108; Yousaf, S., Khan, M. I., Polymorphisms in matrix metalloproteinases MIvIP1 and MMP9 are associated with primary open-angle and angle closure glaucoma in a Pakistani population (2013) Mol V/s, 19, pp. 441-447. , Micheal. S; Osmond, M., Bernier, S. M., Pantcheva, M. B., Collagen and collagen-chondroitin sulfate scaffolds with uniaxially aligned pores for the biomimetic, three dimensional culture of trabecular meshwork cells (2017) Biotechnol Bioeng, 114, pp. 915-923; Reissig, K., Schonfeld, P., Repeated H202 exposure drives cell cycle progression in an in vitro model of ulcerative colitis (2013) J Cell Mol Med, 17, pp. 1619-1631. , Poehlmann. A; Seydel, A., Camoirano, A., Oxidative damage and autophagy in the human trabecular meshwork as related with ageing (2014) PLoS One, 9, p. e98106. , Pulliero. A); Izzotti, A., Focus on molecular events in the anterior chamber leading to glaucoma (2014) Cell Mol Lfe SeE, 71, pp. 2197-2218. , Sacc 1/2, S. C. and; Gandolfi, S., Bagnis, A., From DNA damage to functional changes of the trabecular meshwork in aging and glaucoma (2016) Ageing Res Rev, 29, pp. 26-41. , Sacc 1/2, S. C., (a); Gandolfi, S., Bagnis, A., The outflow pathway: A tissue with morphological and functional unity (2016) J Cell Physiol, 231, pp. 1876-1893. , Sacc 1/2, S. C., (b); Corazza, P., Substances of interest that support glaucoma therapy (2019) Nutrients, 11, p. 239. , Sacc 1/2, S. C., Gandoffi. S. et al; Singh, D., Srivastava, S. K., Chaudhuri, T. K., Multifaceted role of matrix metalloproteinases (MMPs) (2015) Front Mol Biosci, 2, p. 19; Deutscher, M. P., Efficient mammalian protein synthesis requires an intact F-actin system (1997) JBIo! Chem, 272, pp. 24980-24986. , Stapulionis. R., Kolli. S. and; Struebing, F. L., What animal models can tell us about glaucoma (2015) ProgMo!Biol Traits! Sd, 134, pp. 365-380. , and Geisert. E. E; Ripandelli, O., Potential regulatory molecules in the human trabecular meshwork of patients with glaucoma: Immunohistochemical profile of a number of inflammatory cytokines (2015) Mo! Med Rep, 11, pp. 1384-1390. , Pacella. E Taurone. S; Li, X., Wong, T. Y., Global prevalence of glaucoma and projections of glaucoma burden through 2040: A systematic review and meta-analysis (2014) Ophthalmology, 121, pp. 2081-2090. , Tham. Y.-C., l0.10l6/j.ophtha.2014.05.013; Ucciferri, N., Aliluwalia, A., Allomelric scaling and cell ratios in multi-organ in vitro models of human metabolism (2014) Front Bloeng Biotechno, 2, p. 74. , Sbrana. T. and; TNFR2: A novel target for cancer immunotherapy (2017) Trends Mo! Med, 23 (103), pp. 7-1046. , Vanamee. E. S. and Faustman. D. L; De Preter, K., Pattyn, F., Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes (2002) Genome Biol, 3. , researchoO34. 1; Vernazza, S., Tirendi, S., Scarfi, S., 2D- and 3D-cultures of human trabecular meshwork cells: A preliminary assessment of an in vitro model for glaucoma study (2019) PLoS One, 14, p. e0221942; Waduthanthri, K. D., He, Y., Montemagno, C., An injectable peptide hydrogel for reconstruction of the human trabecular meshwork (2019) Acta Biomater, 100, pp. 244-254; Wang, J., Hanis, A., Prendes, M. A., Targeting transforming growth factor-n signaling in primary open- angle glaucoma (2017) J Glaucoma, 26, pp. 390-395; Wang, N., Chintala, S. K., Fini, lvi. E., Activation of a tissue-specific stress response in the aqueous outflow pathway of the eye defines the glaucoma disease phenotype (2001) Nat Med, 7, pp. 304-309; Xiang, Y., Li, B., Li, G.-G., Effects of endothelin-1 on the cytoskeleton protein F-actin of human trabecular meshwork cells in vitro (2010) mt j Ophthamo, 3, pp. 61-63; Zahir, N., Weaver, V. M., Death in the third dimension: Apoptosis regulation and tissue architecture (2004) Curr Opin GenetDev, 14, pp. 71-80; Zhao, J., Wang, S., Zhong, W., Oxidative stress in the trabecular meshwork (Review) (2016) mt J Mo! Med, 38, pp. 995-1002
PY - 2020
Y1 - 2020
N2 - Glaucoma is the second leading cause of blindness worldwide. Currently, glaucoma treatments aim to lower intraocular pressure by decreasing aqueous humor production or increasing aqueous humor outflow through pharmacological approaches or trabeculectomy. The lack of an effective cure requires new therapeutic strategies. We compared the biological responses of a three-dimensional trabecular meshwork model with or without perfusion bioreactor technology to better understand the early molecular changes induced by prolonged oxidative stress conditions induced by repeated daily peroxide exposure. We used standard 3D cultures of trabecular meshwork cells in Matrigel cultured under either static and dynamic conditions for one week. We studied changes in F-actin expression and organization in the cells, cellular metabolic activity, proinflammatory gene expression, expression of pro- and anti-apoptotic proteins, PARP-1 cleavage, and NFKB activation in the model. We demonstrate that the dynamic conditions improve the adaptive behavior of 3D trabecular meshwork cultures to chronic oxidative stress via offsetting pathway activation. © 2020 ALTEX Edition. All rights reserved.
AB - Glaucoma is the second leading cause of blindness worldwide. Currently, glaucoma treatments aim to lower intraocular pressure by decreasing aqueous humor production or increasing aqueous humor outflow through pharmacological approaches or trabeculectomy. The lack of an effective cure requires new therapeutic strategies. We compared the biological responses of a three-dimensional trabecular meshwork model with or without perfusion bioreactor technology to better understand the early molecular changes induced by prolonged oxidative stress conditions induced by repeated daily peroxide exposure. We used standard 3D cultures of trabecular meshwork cells in Matrigel cultured under either static and dynamic conditions for one week. We studied changes in F-actin expression and organization in the cells, cellular metabolic activity, proinflammatory gene expression, expression of pro- and anti-apoptotic proteins, PARP-1 cleavage, and NFKB activation in the model. We demonstrate that the dynamic conditions improve the adaptive behavior of 3D trabecular meshwork cultures to chronic oxidative stress via offsetting pathway activation. © 2020 ALTEX Edition. All rights reserved.
KW - caspase 3
KW - collagen type 1
KW - collagen type 1 alpha 1
KW - complementary DNA
KW - cytochrome c
KW - gelatinase B
KW - immunoglobulin enhancer binding protein
KW - interleukin 1alpha
KW - interleukin 1beta
KW - interleukin 6
KW - interstitial collagenase
KW - matrigel
KW - nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
KW - peroxide
KW - protein Bax
KW - protein bcl 2
KW - protein p53
KW - second mitochondrial activator of caspase
KW - stromelysin
KW - synaptotagmin I
KW - transforming growth factor beta2
KW - tumor necrosis factor
KW - ubiquitin C
KW - unclassified drug
KW - ubiquitin
KW - antiapoptotic activity
KW - apoptosis assay
KW - Article
KW - chronic stress
KW - gene expression
KW - glaucoma
KW - human
KW - human cell
KW - immunoreactivity
KW - in vitro study
KW - metabolic activity assay
KW - oxidative stress
KW - polyacrylamide gel electrophoresis
KW - protein expression
KW - real time polymerase chain reaction
KW - resazurin assay
KW - RNA extraction
KW - three dimensional cell culture
KW - trabecular meshwork
KW - Western blotting
KW - adaptive behavior
KW - cell culture technique
KW - cell growth
KW - confocal microscopy
KW - controlled study
KW - cytokine production
KW - down regulation
KW - flow cytometry
KW - genetic transfection
KW - signal transduction
KW - upregulation
U2 - 10.14573/altex.1909262
DO - 10.14573/altex.1909262
M3 - Article
VL - 37
SP - 265
EP - 274
JO - Altex
JF - Altex
SN - 1868-596X
IS - 2
ER -