An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells

Stefania Pagani, Paola Torricelli, Francesca Veronesi, Francesca Salamanna, Simona Cepollaro, Milena Fini

Research output: Contribution to journalArticle

Abstract

The dynamic metabolism and the numerous roles of bone tissue necessitate a suitable in vitro model to represent them. In order to investigate the interaction among the several cell types composing bone microenvironment, we studied a tri-culture model including human osteoblasts (OBs), osteoclasts (OCs), and endothelial cells (HUVEC). While OBs are essential for bone deposition and OCs for bone resorption, the vasculature is necessary to provide growth factors, nutrients, and oxygen in the mature tissue. The results of this study showed a strong mutual influence between OBs, OCs, and HUVEC in term of proliferation, viability, and activity (release of ALP, Coll I, OPG, RANKL, VEGF, CTSK, TGFβ, and IL-6). The behavior of the single cultures demonstrated to be different compared to the bi- or tri-cultures and depending on the cell types involved: the coexistence of OBs and OCs stimulated the synthetic activity of both cell types, while the presence of HUVEC induced a stimulating role for OBs but mainly an inhibitory effect for OC. In addition, evidence of the effects of OBs and OCs on HUVEC is highlighted by their morphology: regular and able to "sketch" little vessels in presence of OBs, more disorganized and heterogeneous in presence of OCs. Taken together, these observations well characterize an advanced cellular model to be used as starting point for mimicking bone microenvironment in vivo, thus reducing the use of animals in the preclinical phase and offering a more reliable tool to test new and innovative biomaterials.
Original languageEnglish
Pages (from-to)291-301
Number of pages11
JournalJournal of Cellular Physiology
Volume233
Issue number1
DOIs
Publication statusPublished - Jan 2018

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Osteoblasts
Endothelial cells
Osteoclasts
Cell culture
Endothelial Cells
Bone
Bone and Bones
Tissue
Biocompatible Materials
Bone Resorption
Metabolism
Vascular Endothelial Growth Factor A
Nutrients
Interleukin-6
Intercellular Signaling Peptides and Proteins
Animals
Oxygen
Food

Keywords

  • bone cell interactions
  • endothelial cells
  • osteoblasts
  • osteoclasts
  • tri-culture model

Cite this

An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. / Pagani, Stefania; Torricelli, Paola; Veronesi, Francesca; Salamanna, Francesca; Cepollaro, Simona; Fini, Milena.

In: Journal of Cellular Physiology, Vol. 233, No. 1, 01.2018, p. 291-301.

Research output: Contribution to journalArticle

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