An altered redox balance mediates the hypersensitivity of Cockayne syndrome primary fibroblasts to oxidative stress

Barbara Pascucci, Tiziana Lemma, Egidio Iorio, Sara Giovannini, Bruno Vaz, Ivano Iavarone, Angelo Calcagnile, Laura Narciso, Paolo Degan, Franca Podo, Vera Roginskya, Bratislav M. Janjic, Bennett Van Houten, Miria Stefanini, Eugenia Dogliotti, Mariarosaria D'Errico

Research output: Contribution to journalArticlepeer-review


Cockayne syndrome (CS) is a rare hereditary multisystem disease characterized by neurological and development impairment, and premature aging. Cockayne syndrome cells are hypersensitive to oxidative stress, but the molecular mechanisms involved remain unresolved. Here we provide the first evidence that primary fibroblasts derived from patients with CS-A and CS-B present an altered redox balance with increased steady-state levels of intracellular reactive oxygen species (ROS) and basal and induced DNA oxidative damage, loss of the mitochondrial membrane potential, and a significant decrease in the rate of basal oxidative phosphorylation. The Na/K-ATPase, a relevant target of oxidative stress, is also affected with reduced transcription in CS fibroblasts and normal protein levels restored upon complementation with wild-type genes. High-resolution magnetic resonance spectroscopy revealed a significantly perturbed metabolic profile in CS-A and CS-B primary fibroblasts compared with normal cells in agreement with increased oxidative stress and alterations in cell bioenergetics. The affected processes include oxidative metabolism, glycolysis, choline phospholipid metabolism, and osmoregulation. The alterations in intracellular ROS content, oxidative DNA damage, and metabolic profile were partially rescued by the addition of an antioxidant in the culture medium suggesting that the continuous oxidative stress that characterizes CS cells plays a causative role in the underlying pathophysiology. The changes of oxidative and energy metabolism offer a clue for the clinical features of patients with CS and provide novel tools valuable for both diagnosis and therapy.

Original languageEnglish
Pages (from-to)520-529
Number of pages10
JournalAging Cell
Issue number3
Publication statusPublished - Jun 2012


  • Cockayne syndrome
  • DNA oxidation
  • Mitochondrial alteration
  • Oxidative metabolism
  • Oxidative phosphorylation
  • ROS levels

ASJC Scopus subject areas

  • Cell Biology
  • Ageing


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