An alternately spliced mRNA encoding a new soluble form of CD95 is induced in dog cardiomyocytes during ischemia and reperfusion

N. K. Qssina; A. Cannas; R. A. Fitxpatrirk; S. K. Umansky; M. C. Kiefer

An alternately spliced mRNA encoding a new soluble form of CD95 is induced in dog cardiomyocytes during ischemia and reperfusion

N. K. Qssina, A. Cannas, R. A. Fitxpatrirk, S. K. Umansky, M. C. Kiefer

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

CD95 (FAS/APO1) is an apoptosis-signaling roll surface receptor hehme ing to the TNK/NCJF receptor family. We have previously shown that Ci)l'.r> transcription i, induced in dog rtiyocardiocytes after ischemia and reperfuMnn. A cDNA library was prepared froni dog cardiomyocytes after in vivo ischemia and reperfusion- and a ('1)9') d)\A was isolated. Sequence analvsis "mdi(ated that the cDNA contained an unsplired intron 5, with an in-frame >top codon 19 nucleotides downstream from the splice donor site of the "nitron J'hc n suiting cDN A encodes a 1 Tli ami no acid protein representing a pu t at ive solnbh1 extracellular (FC) ('1)95 receptor. Northern hybridization using inlron 5 I).\.\ as a probe showed that this form of mRNA is upregulated in myocardial <

Original language	English
Journal	FASEB Journal
Volume	11
Issue number	9
Publication status	Published - 1997

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Cell Biology

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author = "Qssina, {N. K.} and A. Cannas and Fitxpatrirk, {R. A.} and Umansky, {S. K.} and Kiefer, {M. C.}",

year = "1997",

language = "English",

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T1 - An alternately spliced mRNA encoding a new soluble form of CD95 is induced in dog cardiomyocytes during ischemia and reperfusion

AU - Qssina, N. K.

AU - Cannas, A.

AU - Fitxpatrirk, R. A.

AU - Umansky, S. K.

AU - Kiefer, M. C.

PY - 1997

Y1 - 1997

N2 - CD95 (FAS/APO1) is an apoptosis-signaling roll surface receptor hehme ing to the TNK/NCJF receptor family. We have previously shown that Ci)l'.r> transcription i, induced in dog rtiyocardiocytes after ischemia and reperfuMnn. A cDNA library was prepared froni dog cardiomyocytes after in vivo ischemia and reperfusion- and a ('1)9') d)\A was isolated. Sequence analvsis "mdi(ated that the cDNA contained an unsplired intron 5, with an in-frame >top codon 19 nucleotides downstream from the splice donor site of the "nitron J'hc n suiting cDN A encodes a 1 Tli ami no acid protein representing a pu t at ive solnbh1 extracellular (FC) ('1)95 receptor. Northern hybridization using inlron 5 I).\.\ as a probe showed that this form of mRNA is upregulated in myocardial <

AB - CD95 (FAS/APO1) is an apoptosis-signaling roll surface receptor hehme ing to the TNK/NCJF receptor family. We have previously shown that Ci)l'.r> transcription i, induced in dog rtiyocardiocytes after ischemia and reperfuMnn. A cDNA library was prepared froni dog cardiomyocytes after in vivo ischemia and reperfusion- and a ('1)9') d)\A was isolated. Sequence analvsis "mdi(ated that the cDNA contained an unsplired intron 5, with an in-frame >top codon 19 nucleotides downstream from the splice donor site of the "nitron J'hc n suiting cDN A encodes a 1 Tli ami no acid protein representing a pu t at ive solnbh1 extracellular (FC) ('1)95 receptor. Northern hybridization using inlron 5 I).\.\ as a probe showed that this form of mRNA is upregulated in myocardial <

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An alternately spliced mRNA encoding a new soluble form of CD95 is induced in dog cardiomyocytes during ischemia and reperfusion

Abstract

ASJC Scopus subject areas

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