An alternately spliced mRNA encoding a new soluble form of CD95 is induced in dog cardiomyocytes during ischemia and reperfusion

CD95 (FAS/APO1) is an apoptosis-signaling roll surface receptor hehme ing to the TNK/NCJF receptor family. We have previously shown that Ci)l'.r> transcription i, induced in dog rtiyocardiocytes after ischemia and reperfuMnn. A cDNA library was prepared froni dog cardiomyocytes after in vivo ischemia and reperfusion- and a ('1)9') d)\A was isolated. Sequence analvsis "mdi(ated that the cDNA contained an unsplired intron 5, with an in-frame >top codon 19 nucleotides downstream from the splice donor site of the "nitron J'hc n suiting cDN A encodes a 1 Tli ami no acid protein representing a pu t at ive solnbh1 extracellular (FC) ('1)95 receptor. Northern hybridization using inlron 5 I).\.\ as a probe showed that this form of mRNA is upregulated in myocardial <