An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells

Manuele Castelnuovo, Sara Massone, Roberta Tasso, Gloria Fiorino, Monica Gatti, Mauro Robello, Elena Gatta, Audrey Berger, Katharina Strub, Tullio Florio, Giorgio Dieci, Ranieri Cancedda, Aldo Pagano

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Abstract

Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that the synthesis of a pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes, and a dramatic reduction of the tumor initiating cell (TIC) fraction in the NB cell population. Notably, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Altogether, these results suggest the induction of NDM29 expression as possible treatment to increase cancer cells vulnerability to therapeutics and the measure of its synthesis in NB explants as prognostic factor of this cancer type.

Original languageEnglish
Pages (from-to)4033-4046
Number of pages14
JournalFASEB Journal
Volume24
Issue number10
DOIs
Publication statusPublished - 2010

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Keywords

  • Anticancer drugs
  • ncRNA
  • Neuroblastoma differentiation
  • RNA polymerase III

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Castelnuovo, M., Massone, S., Tasso, R., Fiorino, G., Gatti, M., Robello, M., Gatta, E., Berger, A., Strub, K., Florio, T., Dieci, G., Cancedda, R., & Pagano, A. (2010). An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells. FASEB Journal, 24(10), 4033-4046. https://doi.org/10.1096/fj.10-157032