An amidated carboxymethylcellulose hydrogel for cartilage regeneration

Gemma Leone, Milena Fini, Paola Torricelli, Roberto Giardino, Rolando Barbucci

Research output: Contribution to journalArticlepeer-review

Abstract

An amidic derivative of carboxymethylcellulose was synthesized (CMCA). The new polysaccharide was obtained by converting a large percentage of carboxylic groups (∼50%) of carboxymethylcellulose into amidic groups rendering the macromolecule quite similar to hyaluronan. Then, the polysaccharide (CMCA) was crosslinked. The behavior of CMCA hydrogel towards normal human articular chondrocytes (NHAC) was in vitro studied monitoring the cell proliferation and synthesis of extra cellular matrix (ECM) components and compared with a hyaluronan based hydrogel (Hyal). An extracellular matrix rich in cartilage-specific collagen and proteoglycans was secreted in the presence of hydrogels. The injectability of the new hydrogels was also analysed. An experimental in vivo model was realized to study the effect of CMCA and Hyal hydrogels in the treatment of surgically created partial thickness chondral defects in the rabbit knee. The preliminary results pointed out that CMCA hydrogel could be considered as a potential compound for cartilage regeneration.

Original languageEnglish
Pages (from-to)2873-2880
Number of pages8
JournalJournal of Materials Science: Materials in Medicine
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 2008

ASJC Scopus subject areas

  • Biophysics
  • Chemical Engineering(all)
  • Bioengineering

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