An analysis in human lymphomas of a Jα region involved in a C-MYC Jα translocation; Relationship with TCR alpha, beta, and gamma rearrangements

Anna Villa, Maria Grazia Sacco, Gaetano Cairo, Ida Biunno, Danièle Mathieu-Mahul, Jacques Larsen, Paolo Vezzoni

Research output: Contribution to journalArticle

Abstract

The D14S7 locus defines the breakpoint on chromosome 14 of a t (8;14) (q24;q11) present in the T-cell line KE37-R in which DI4S7 sequences translocate 3′ to the c-myc oncogene. It has been shown previously that DI4S7 rearranges specifically in some but not all T cell clones and in the present study we investigated the frequency and specificity of its rearrangements in human fresh lymphoma samples.DI4S7 rearrangements were extremely specific since they were detected in 3 out of 5 T-cell lymphoma samples positive for TCRβandγbut not in 17 miscellaneous non-T lymphomas, 4 non neoplastic lymphnodes as well as unstimulated and activated polyclonal T-cells. Most of the rearrangements were in the form of deletions that appear to involve large pieces of DNA since the segments detected by a Vαprobe were also deleted. Rearrangement of DI4S7 and Vα regions were detected in lymphomas with a cortical thymocyte phenotype, demonstrating that they appear quite early in the differentiation of T cells.

Original languageEnglish
Pages (from-to)550-558
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume154
Issue number2
DOIs
Publication statusPublished - Jul 29 1988

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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