Abstract
The D14S7 locus defines the breakpoint on chromosome 14 of a t (8;14) (q24;q11) present in the T-cell line KE37-R in which DI4S7 sequences translocate 3′ to the c-myc oncogene. It has been shown previously that DI4S7 rearranges specifically in some but not all T cell clones and in the present study we investigated the frequency and specificity of its rearrangements in human fresh lymphoma samples.DI4S7 rearrangements were extremely specific since they were detected in 3 out of 5 T-cell lymphoma samples positive for TCRβandγbut not in 17 miscellaneous non-T lymphomas, 4 non neoplastic lymphnodes as well as unstimulated and activated polyclonal T-cells. Most of the rearrangements were in the form of deletions that appear to involve large pieces of DNA since the segments detected by a Vαprobe were also deleted. Rearrangement of DI4S7 and Vα regions were detected in lymphomas with a cortical thymocyte phenotype, demonstrating that they appear quite early in the differentiation of T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 550-558 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 154 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 29 1988 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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An analysis in human lymphomas of a Jα region involved in a C-MYC Jα translocation; Relationship with TCR alpha, beta, and gamma rearrangements. / Villa, Anna; Sacco, Maria Grazia; Cairo, Gaetano; Biunno, Ida; Mathieu-Mahul, Danièle; Larsen, Jacques; Vezzoni, Paolo.
In: Biochemical and Biophysical Research Communications, Vol. 154, No. 2, 29.07.1988, p. 550-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An analysis in human lymphomas of a Jα region involved in a C-MYC Jα translocation; Relationship with TCR alpha, beta, and gamma rearrangements
AU - Villa, Anna
AU - Sacco, Maria Grazia
AU - Cairo, Gaetano
AU - Biunno, Ida
AU - Mathieu-Mahul, Danièle
AU - Larsen, Jacques
AU - Vezzoni, Paolo
PY - 1988/7/29
Y1 - 1988/7/29
N2 - The D14S7 locus defines the breakpoint on chromosome 14 of a t (8;14) (q24;q11) present in the T-cell line KE37-R in which DI4S7 sequences translocate 3′ to the c-myc oncogene. It has been shown previously that DI4S7 rearranges specifically in some but not all T cell clones and in the present study we investigated the frequency and specificity of its rearrangements in human fresh lymphoma samples.DI4S7 rearrangements were extremely specific since they were detected in 3 out of 5 T-cell lymphoma samples positive for TCRβandγbut not in 17 miscellaneous non-T lymphomas, 4 non neoplastic lymphnodes as well as unstimulated and activated polyclonal T-cells. Most of the rearrangements were in the form of deletions that appear to involve large pieces of DNA since the segments detected by a Vαprobe were also deleted. Rearrangement of DI4S7 and Vα regions were detected in lymphomas with a cortical thymocyte phenotype, demonstrating that they appear quite early in the differentiation of T cells.
AB - The D14S7 locus defines the breakpoint on chromosome 14 of a t (8;14) (q24;q11) present in the T-cell line KE37-R in which DI4S7 sequences translocate 3′ to the c-myc oncogene. It has been shown previously that DI4S7 rearranges specifically in some but not all T cell clones and in the present study we investigated the frequency and specificity of its rearrangements in human fresh lymphoma samples.DI4S7 rearrangements were extremely specific since they were detected in 3 out of 5 T-cell lymphoma samples positive for TCRβandγbut not in 17 miscellaneous non-T lymphomas, 4 non neoplastic lymphnodes as well as unstimulated and activated polyclonal T-cells. Most of the rearrangements were in the form of deletions that appear to involve large pieces of DNA since the segments detected by a Vαprobe were also deleted. Rearrangement of DI4S7 and Vα regions were detected in lymphomas with a cortical thymocyte phenotype, demonstrating that they appear quite early in the differentiation of T cells.
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U2 - 10.1016/0006-291X(88)90175-1
DO - 10.1016/0006-291X(88)90175-1
M3 - Article
C2 - 3401221
AN - SCOPUS:0023736944
VL - 154
SP - 550
EP - 558
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -