An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1

Shima Salehi, Behzad Mansoori, Ali Mohammadi, Sadaf Davoudian, Seyed Mohammad Hossein Musavi Shenas, Neda Shajari, Jafar Majidi, Behzad Baradaran

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND: Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.

METHODS: Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.

RESULTS: The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.

CONCLUSION: These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.

Original languageEnglish
Pages (from-to)545-550
Number of pages6
JournalBiomedicine and Pharmacotherapy
Volume96
DOIs
Publication statusPublished - Dec 2017
Externally publishedYes

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Snails
Urinary Bladder Neoplasms
Cell Line
Small Interfering RNA
Cell Migration Assays
In Situ Nick-End Labeling
Vimentin
Cadherins
Matrix Metalloproteinases
Wound Healing
Transfection
Neoplasms
Cell Death
Western Blotting
Gene Expression
Polymerase Chain Reaction
Survival
Therapeutics
Proteins

Cite this

Salehi, S., Mansoori, B., Mohammadi, A., Davoudian, S., Musavi Shenas, S. M. H., Shajari, N., ... Baradaran, B. (2017). An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1. Biomedicine and Pharmacotherapy, 96, 545-550. https://doi.org/10.1016/j.biopha.2017.10.044

An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1. / Salehi, Shima; Mansoori, Behzad; Mohammadi, Ali; Davoudian, Sadaf; Musavi Shenas, Seyed Mohammad Hossein; Shajari, Neda; Majidi, Jafar; Baradaran, Behzad.

In: Biomedicine and Pharmacotherapy, Vol. 96, 12.2017, p. 545-550.

Research output: Contribution to journalArticle

Salehi, S, Mansoori, B, Mohammadi, A, Davoudian, S, Musavi Shenas, SMH, Shajari, N, Majidi, J & Baradaran, B 2017, 'An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1', Biomedicine and Pharmacotherapy, vol. 96, pp. 545-550. https://doi.org/10.1016/j.biopha.2017.10.044
Salehi, Shima ; Mansoori, Behzad ; Mohammadi, Ali ; Davoudian, Sadaf ; Musavi Shenas, Seyed Mohammad Hossein ; Shajari, Neda ; Majidi, Jafar ; Baradaran, Behzad. / An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1. In: Biomedicine and Pharmacotherapy. 2017 ; Vol. 96. pp. 545-550.
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abstract = "BACKGROUND: Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.METHODS: Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.RESULTS: The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.CONCLUSION: These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.",
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AU - Musavi Shenas, Seyed Mohammad Hossein

AU - Shajari, Neda

AU - Majidi, Jafar

AU - Baradaran, Behzad

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N2 - BACKGROUND: Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.METHODS: Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.RESULTS: The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.CONCLUSION: These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.

AB - BACKGROUND: Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.METHODS: Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.RESULTS: The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.CONCLUSION: These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.

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