An anti-inflammatory protein secreted from the rat seminal vesicle epithelium inhibits the synthesis of platelet-activating factor and the release of arachidonic acid and prostacyclin

G. Camussi, C. Tetta, F. Bussolino, S. Metafora, G. Peluso, C. Esposito, R. Porta

Research output: Contribution to journalArticle

Abstract

Platelet-activating factor (PAF), a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, is a mediator of inflammation and endotoxic shock produced by a variety of stimulated cells. Since the main biosynthetic pathway of PAF involves acetylation of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) generated from 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine by phospholipase A2, we suggest a general physiological role played by steroid-induced anti-(phospholipase A2) proteins in the modulation of PAF synthesis. The results of the present study support this hypothesis since an androgen-induced anti-inflammatory protein, SV-IV, secreted from rat seminal vesicles, inhibits PAF synthesis in stimulated polymorphonuclear neutrophils, macrophages and endothelial cells. SV-IV impairs PAF synthesis by inhibiting the activation of phospholipase A2, that also results in the inhibition of arachidonic acid and prostacyclin release, and of acetyl-CoA:lyso-PAF acetyltransferase.

Original languageEnglish
Pages (from-to)481-485
Number of pages5
JournalEuropean Journal of Biochemistry
Volume192
Issue number2
DOIs
Publication statusPublished - 1990

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Seminal Vesicles
Platelet Activating Factor
Epoprostenol
Arachidonic Acid
Rats
Anti-Inflammatory Agents
Epithelium
Phosphorylcholine
Phospholipases A2
1-alkylglycerophosphocholine acetyltransferase
Proteins
Acetylation
Inflammation Mediators
Macrophages
Biosynthetic Pathways
Endothelial cells
Septic Shock
Androgens
Neutrophils
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

An anti-inflammatory protein secreted from the rat seminal vesicle epithelium inhibits the synthesis of platelet-activating factor and the release of arachidonic acid and prostacyclin. / Camussi, G.; Tetta, C.; Bussolino, F.; Metafora, S.; Peluso, G.; Esposito, C.; Porta, R.

In: European Journal of Biochemistry, Vol. 192, No. 2, 1990, p. 481-485.

Research output: Contribution to journalArticle

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AU - Bussolino, F.

AU - Metafora, S.

AU - Peluso, G.

AU - Esposito, C.

AU - Porta, R.

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