An archipelago of CpG islands in Xq28: Identification and fine mapping of 20 new CpG islands of the human X chromosome

Elena Maestrini, Filippo Tamanini, Petra Kioschis, Elisabeth Gimbo, Paola Marinelli, Carla Tribioli, Michele D'Urso, Giuseppe Palmieri, Annemarie Poustka, Daniela Toniolo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

19 probes for CpG islands, mapping to Xq28, have been used as probes to construct a physical map of genes of this band of the human X chromosome. A total of 22 CpG islands have been precisely mapped in respect to known loci along the 9-10 Mb of Xq28. The fine mapping of such a large number of CpG islands has demonstrated that also in gene rich Giemsa light bands, like Xq28, gene distribution is non uniform: the CpG islands are clustered in the distal portion of the band in a 2 Mb region between the G6PD gene and the DXS15 locus. Moreover, 16 CpG islands were found between the G6PD and the RCP/GCP genes, a region of DNA of only about 300 kb. If this structural organization has a biological function it has yet to be determined. However, the isolation of large genomic regions enriched in gene sequences and the availability of cosmid or YAC contigs will provide the means to test the significance of such gene organization, as well as the material for large sequencing projects and gene search, for the identification of candidate genes for inherited disorders mapped to Xq28 and for comparative mapping.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalHuman Molecular Genetics
Volume1
Issue number4
Publication statusPublished - Jul 1992

Fingerprint

Chromosomes, Human, X
CpG Islands
Chromosomes
Chromosome
Genes
Gene
Locus
Probe
Cosmids
Human
Genetic Association Studies
Sequencing
Isolation
Genomics
Disorder
DNA
Availability

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)

Cite this

Maestrini, E., Tamanini, F., Kioschis, P., Gimbo, E., Marinelli, P., Tribioli, C., ... Toniolo, D. (1992). An archipelago of CpG islands in Xq28: Identification and fine mapping of 20 new CpG islands of the human X chromosome. Human Molecular Genetics, 1(4), 275-280.

An archipelago of CpG islands in Xq28 : Identification and fine mapping of 20 new CpG islands of the human X chromosome. / Maestrini, Elena; Tamanini, Filippo; Kioschis, Petra; Gimbo, Elisabeth; Marinelli, Paola; Tribioli, Carla; D'Urso, Michele; Palmieri, Giuseppe; Poustka, Annemarie; Toniolo, Daniela.

In: Human Molecular Genetics, Vol. 1, No. 4, 07.1992, p. 275-280.

Research output: Contribution to journalArticle

Maestrini, E, Tamanini, F, Kioschis, P, Gimbo, E, Marinelli, P, Tribioli, C, D'Urso, M, Palmieri, G, Poustka, A & Toniolo, D 1992, 'An archipelago of CpG islands in Xq28: Identification and fine mapping of 20 new CpG islands of the human X chromosome', Human Molecular Genetics, vol. 1, no. 4, pp. 275-280.
Maestrini E, Tamanini F, Kioschis P, Gimbo E, Marinelli P, Tribioli C et al. An archipelago of CpG islands in Xq28: Identification and fine mapping of 20 new CpG islands of the human X chromosome. Human Molecular Genetics. 1992 Jul;1(4):275-280.
Maestrini, Elena ; Tamanini, Filippo ; Kioschis, Petra ; Gimbo, Elisabeth ; Marinelli, Paola ; Tribioli, Carla ; D'Urso, Michele ; Palmieri, Giuseppe ; Poustka, Annemarie ; Toniolo, Daniela. / An archipelago of CpG islands in Xq28 : Identification and fine mapping of 20 new CpG islands of the human X chromosome. In: Human Molecular Genetics. 1992 ; Vol. 1, No. 4. pp. 275-280.
@article{c8407e8fe6c84a2ab556a5e1639d89f8,
title = "An archipelago of CpG islands in Xq28: Identification and fine mapping of 20 new CpG islands of the human X chromosome",
abstract = "19 probes for CpG islands, mapping to Xq28, have been used as probes to construct a physical map of genes of this band of the human X chromosome. A total of 22 CpG islands have been precisely mapped in respect to known loci along the 9-10 Mb of Xq28. The fine mapping of such a large number of CpG islands has demonstrated that also in gene rich Giemsa light bands, like Xq28, gene distribution is non uniform: the CpG islands are clustered in the distal portion of the band in a 2 Mb region between the G6PD gene and the DXS15 locus. Moreover, 16 CpG islands were found between the G6PD and the RCP/GCP genes, a region of DNA of only about 300 kb. If this structural organization has a biological function it has yet to be determined. However, the isolation of large genomic regions enriched in gene sequences and the availability of cosmid or YAC contigs will provide the means to test the significance of such gene organization, as well as the material for large sequencing projects and gene search, for the identification of candidate genes for inherited disorders mapped to Xq28 and for comparative mapping.",
author = "Elena Maestrini and Filippo Tamanini and Petra Kioschis and Elisabeth Gimbo and Paola Marinelli and Carla Tribioli and Michele D'Urso and Giuseppe Palmieri and Annemarie Poustka and Daniela Toniolo",
year = "1992",
month = "7",
language = "English",
volume = "1",
pages = "275--280",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - An archipelago of CpG islands in Xq28

T2 - Identification and fine mapping of 20 new CpG islands of the human X chromosome

AU - Maestrini, Elena

AU - Tamanini, Filippo

AU - Kioschis, Petra

AU - Gimbo, Elisabeth

AU - Marinelli, Paola

AU - Tribioli, Carla

AU - D'Urso, Michele

AU - Palmieri, Giuseppe

AU - Poustka, Annemarie

AU - Toniolo, Daniela

PY - 1992/7

Y1 - 1992/7

N2 - 19 probes for CpG islands, mapping to Xq28, have been used as probes to construct a physical map of genes of this band of the human X chromosome. A total of 22 CpG islands have been precisely mapped in respect to known loci along the 9-10 Mb of Xq28. The fine mapping of such a large number of CpG islands has demonstrated that also in gene rich Giemsa light bands, like Xq28, gene distribution is non uniform: the CpG islands are clustered in the distal portion of the band in a 2 Mb region between the G6PD gene and the DXS15 locus. Moreover, 16 CpG islands were found between the G6PD and the RCP/GCP genes, a region of DNA of only about 300 kb. If this structural organization has a biological function it has yet to be determined. However, the isolation of large genomic regions enriched in gene sequences and the availability of cosmid or YAC contigs will provide the means to test the significance of such gene organization, as well as the material for large sequencing projects and gene search, for the identification of candidate genes for inherited disorders mapped to Xq28 and for comparative mapping.

AB - 19 probes for CpG islands, mapping to Xq28, have been used as probes to construct a physical map of genes of this band of the human X chromosome. A total of 22 CpG islands have been precisely mapped in respect to known loci along the 9-10 Mb of Xq28. The fine mapping of such a large number of CpG islands has demonstrated that also in gene rich Giemsa light bands, like Xq28, gene distribution is non uniform: the CpG islands are clustered in the distal portion of the band in a 2 Mb region between the G6PD gene and the DXS15 locus. Moreover, 16 CpG islands were found between the G6PD and the RCP/GCP genes, a region of DNA of only about 300 kb. If this structural organization has a biological function it has yet to be determined. However, the isolation of large genomic regions enriched in gene sequences and the availability of cosmid or YAC contigs will provide the means to test the significance of such gene organization, as well as the material for large sequencing projects and gene search, for the identification of candidate genes for inherited disorders mapped to Xq28 and for comparative mapping.

UR - http://www.scopus.com/inward/record.url?scp=0026894185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026894185&partnerID=8YFLogxK

M3 - Article

C2 - 1303198

AN - SCOPUS:0026894185

VL - 1

SP - 275

EP - 280

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

IS - 4

ER -