An artificial model for double mixed agglutination was worked out using baby hamster kidney cells (BHK) from a continuous culture and sheep and guinea-pig erythrocytes as indicators. The rabbit IgG of different allotypes was built into the surface of the BHK cells by bis-diazotized benzidine; the erythrocytes were coated with rabbit antibodies (mainly IgG) to them. The anti-erythrocyte antibodies used had a defined allotypic structure. Reaction between BHK cells with rabbit IgG determinants and the 2 types (sheep and guinea-pig) of indicator cell has been demonstrated.
ASJC Scopus subject areas
- Immunology and Allergy