An assay for simultaneous multiple determinations of peptide binding to MHC class II molecules

A. A. Manfredi, Ha Yuen Mi Ha Yuen, M. A. Raftery, B. M. Conti-Tronconi

Research output: Contribution to journalArticle

Abstract

Binding of positively charged radiolabeled synthetic peptides to human major histocompatibility complex class II DR molecules, purified by affinity chromatography from lymphoblastoid B cell lines of different haplotypes, is rapidly, quantitatively, and specifically assayed by selective adsorption of the complexes between peptide and DR molecules onto DEAE-cellulose paper disks. This assay can be used as a revealing system of the ability of unlabeled test peptides to competitively inhibit the binding between the radiolabeled peptide and the DR molecules, thus measuring the binding of the competitor peptides, irrespective of their charge properties, to different DR molecules.

Original languageEnglish
Pages (from-to)267-273
Number of pages7
JournalAnalytical Biochemistry
Volume211
Issue number2
DOIs
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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