An Automated High Throughput Screening-Compatible Assay to Identify Regulators of Stem Cell Neural Differentiation

Laura Casalino, Dario Magnani, Sandro De Falco, Stefania Filosa, Gabriella Minchiotti, Eduardo J. Patriarca, Dario De Cesare

Research output: Contribution to journalArticlepeer-review

Abstract

The use of Embryonic Stem Cells (ESCs) holds considerable promise both for drug discovery programs and the treatment of degenerative disorders in regenerative medicine approaches. Nevertheless, the successful use of ESCs is still limited by the lack of efficient control of ESC self-renewal and differentiation capabilities. In this context, the possibility to modulate ESC biological properties and to obtain homogenous populations of correctly specified cells will help developing physiologically relevant screens, designed for the identification of stem cell modulators. Here, we developed a high throughput screening-suitable ESC neural differentiation assay by exploiting the Cellmaker robotic platform and demonstrated that neural progenies can be generated from ESCs in complete automation, with high standards of accuracy and reliability. Moreover, we performed a pilot screening providing proof of concept that this assay allows the identification of regulators of ESC neural differentiation in full automation.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalMolecular Biotechnology
DOIs
Publication statusAccepted/In press - 2011

Keywords

  • Automation
  • Cell-based assay
  • Embryonic stem cells
  • HTS
  • Molecular screening
  • Neural differentiation
  • Z-score factor

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Molecular Biology
  • Biotechnology
  • Biochemistry

Fingerprint

Dive into the research topics of 'An Automated High Throughput Screening-Compatible Assay to Identify Regulators of Stem Cell Neural Differentiation'. Together they form a unique fingerprint.

Cite this