An automated system for animal exposure to nitrogen dioxide

Maria Romano, Paolo Pagani, Marina Ferro, Annalaura Erroi, Marco Moggi, Fabio Barozzi, Ugo Dal Molin, Vittorio Alongi, Claudio Zucchi, Mario Salmona

Research output: Contribution to journalArticlepeer-review


We have designed an automated system for controlled exposure of rodents to nitrogen dioxide (NO2). The system consists of 1) two stainless-steel exposure chambers (3.96 m3), for control and treated animals, in which temperature, humidity, and air changes are consistent with current guidelines for rodent housing; 2) a cylinder containing 1% NO2 in N2; 3) a detector connected with a personal computer system that monitors the NO2 concentration, and regulates the NO2 influx, using a closed-loop feedback; and 4) a fan system to distribute the gas uniformly within the chambers. In a typical experiment rats were exposed to 15 ppm of NO2, and changes in the differential count of polymorphonuclear leukocytes, macrophages, and lymphocytes in bronchoalveolar lavage fluid were investigated.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalJournal of Pharmacological and Toxicological Methods
Issue number4
Publication statusPublished - 1994


  • Air pollutants
  • Bronchoalveolar lavage fluid
  • Exposure system
  • Nitrogen dioxide
  • Rodents

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology


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