An automated system for animal exposure to nitrogen dioxide

Maria Romano, Paolo Pagani, Marina Ferro, Annalaura Erroi, Marco Moggi, Fabio Barozzi, Ugo Dal Molin, Vittorio Alongi, Claudio Zucchi, Mario Salmona

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have designed an automated system for controlled exposure of rodents to nitrogen dioxide (NO2). The system consists of 1) two stainless-steel exposure chambers (3.96 m3), for control and treated animals, in which temperature, humidity, and air changes are consistent with current guidelines for rodent housing; 2) a cylinder containing 1% NO2 in N2; 3) a detector connected with a personal computer system that monitors the NO2 concentration, and regulates the NO2 influx, using a closed-loop feedback; and 4) a fan system to distribute the gas uniformly within the chambers. In a typical experiment rats were exposed to 15 ppm of NO2, and changes in the differential count of polymorphonuclear leukocytes, macrophages, and lymphocytes in bronchoalveolar lavage fluid were investigated.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalJournal of Pharmacological and Toxicological Methods
Volume31
Issue number4
DOIs
Publication statusPublished - 1994

Fingerprint

Nitrogen Dioxide
Rodentia
Animals
Lymphocytes
Macrophages
Stainless Steel
Computer Systems
Bronchoalveolar Lavage Fluid
Microcomputers
Humidity
Personal computers
Fans
Rats
Atmospheric humidity
Neutrophils
Computer systems
Gases
Air
Guidelines
Detectors

Keywords

  • Air pollutants
  • Bronchoalveolar lavage fluid
  • Exposure system
  • Nitrogen dioxide
  • Rodents

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

An automated system for animal exposure to nitrogen dioxide. / Romano, Maria; Pagani, Paolo; Ferro, Marina; Erroi, Annalaura; Moggi, Marco; Barozzi, Fabio; Molin, Ugo Dal; Alongi, Vittorio; Zucchi, Claudio; Salmona, Mario.

In: Journal of Pharmacological and Toxicological Methods, Vol. 31, No. 4, 1994, p. 187-190.

Research output: Contribution to journalArticle

Romano, M, Pagani, P, Ferro, M, Erroi, A, Moggi, M, Barozzi, F, Molin, UD, Alongi, V, Zucchi, C & Salmona, M 1994, 'An automated system for animal exposure to nitrogen dioxide', Journal of Pharmacological and Toxicological Methods, vol. 31, no. 4, pp. 187-190. https://doi.org/10.1016/1056-8719(94)90002-7
Romano, Maria ; Pagani, Paolo ; Ferro, Marina ; Erroi, Annalaura ; Moggi, Marco ; Barozzi, Fabio ; Molin, Ugo Dal ; Alongi, Vittorio ; Zucchi, Claudio ; Salmona, Mario. / An automated system for animal exposure to nitrogen dioxide. In: Journal of Pharmacological and Toxicological Methods. 1994 ; Vol. 31, No. 4. pp. 187-190.
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