An automatic method for identifying surface proteins in bacteria: SLEP

Emanuela Giombini, Massimiliano Orsini, Danilo Carrabino, Anna Tramontano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Bacterial infections represent a global health challenge. The identification of novel antibacterial targets for both therapy and vaccination is needed on a constant basis because resistance continues to spread worldwide at an alarming rate. Even infections that were once easy to treat are becoming difficult or, in some cases, impossible to cure. Ideal targets for both therapy and vaccination are bacterial proteins exposed on the surface of the organism, which are often involved in host-pathogen interaction. Their identification can greatly benefit from technologies such as bioinformatics, proteomics and DNA microarrays.Results: Here we describe a pipeline named SLEP (Surface Localization Extracellular Proteins), based on an automated optimal combination and sequence of usage of reliable available tools for the computational identification of the surfome, i.e. of the subset of proteins exposed on the surface of a bacterial cell.Conclusions: The tool not only simplifies the usage of these methods, but it also improves the results by selecting the specifying order and combination of the instruments. The tool is freely available at http://www.caspur.it/slep.

Original languageEnglish
Article number39
JournalBMC Bioinformatics
Volume11
DOIs
Publication statusPublished - Jan 20 2010

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Bacteria
Membrane Proteins
Vaccination
Proteins
Protein
Host-Pathogen Interactions
Therapy
Bacterial Proteins
Infection
Oligonucleotide Array Sequence Analysis
Computational Biology
Bacterial Infections
Proteomics
DNA Microarray
Target
Pathogens
Bioinformatics
Microarrays
Technology
Simplify

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Structural Biology
  • Applied Mathematics

Cite this

An automatic method for identifying surface proteins in bacteria : SLEP. / Giombini, Emanuela; Orsini, Massimiliano; Carrabino, Danilo; Tramontano, Anna.

In: BMC Bioinformatics, Vol. 11, 39, 20.01.2010.

Research output: Contribution to journalArticle

Giombini, Emanuela ; Orsini, Massimiliano ; Carrabino, Danilo ; Tramontano, Anna. / An automatic method for identifying surface proteins in bacteria : SLEP. In: BMC Bioinformatics. 2010 ; Vol. 11.
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