An automatic segmentation method combining an active contour model and a classification technique for detecting polycomb-group proteins in high-throughput microscopy images

Francesco Gregoretti, Elisa Cesarini, Chiara Lanzuolo, Gennaro Oliva, Laura Antonelli

Research output: Contribution to journalArticle

Abstract

The large amount of data generated in biological experiments that rely on advanced microscopy can be handled only with automated image analysis. Most analyses require a reliable cell image segmentation eventually capable of detecting subcellular structures. We present an automatic segmentation method to detect Polycomb group (PcG) proteins areas isolated from nuclei regions in high-resolution fluorescent cell image stacks. It combines two segmentation algorithms that use an active contour model and a classification technique serving as a tool to better understand the subcellular three-dimensional distribution of PcG proteins in live cell image sequences. We obtained accurate results throughout several cell image datasets, coming from different cell types and corresponding to different fluorescent labels, without requiring elaborate adjustments to each dataset.

Original languageEnglish
Pages (from-to)181-197
Number of pages17
JournalMethods in Molecular Biology
Volume1480
DOIs
Publication statusPublished - 2016

Keywords

  • Cell segmentation
  • Fluorescence microscopy
  • High-throughput imaging
  • Polycomb group of proteins
  • Thresholding techniques
  • Variational models

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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