An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster, Desire Di Silvio, Aarthi Devarajan, Paolo Bigini, Edoardo Micotti, Chiara Giudice, Mario Salmona, Grant N. Wheeler, Victoria Sherwood, Francesca Baldelli Bombelli

Research output: Contribution to journalArticle

Abstract

Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

Original languageEnglish
Pages (from-to)643-656
Number of pages14
JournalNanomedicine
Volume11
Issue number6
DOIs
Publication statusPublished - Mar 1 2016

Keywords

  • cytotoxicity
  • in vitro-in vivo correlations
  • nanomaterials
  • nanomedicine
  • nanotoxicity
  • Xenopus laevis

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

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  • Cite this

    Webster, C. A., Di Silvio, D., Devarajan, A., Bigini, P., Micotti, E., Giudice, C., Salmona, M., Wheeler, G. N., Sherwood, V., & Bombelli, F. B. (2016). An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment. Nanomedicine, 11(6), 643-656. https://doi.org/10.2217/nnm.15.219