An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster, Desire Di Silvio, Aarthi Devarajan, Paolo Bigini, Edoardo Micotti, Chiara Giudice, Mario Salmona, Grant N. Wheeler, Victoria Sherwood, Francesca Baldelli Bombelli

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

Original languageEnglish
Pages (from-to)643-656
Number of pages14
JournalNanomedicine
Volume11
Issue number6
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Nanostructures
Nanostructured materials
Nanoparticles
Toxicity
Vertebrates
vertebrate
Xenopus
safety
toxicity
Safety
embryo
Magnetite Nanoparticles
Cytotoxicity
Magnetite
Assays
Hazards
hazard assessment
Screening
abnormality
Embryonic Structures

Keywords

  • cytotoxicity
  • in vitro-in vivo correlations
  • nanomaterials
  • nanomedicine
  • nanotoxicity
  • Xenopus laevis

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

An early developmental vertebrate model for nanomaterial safety : Bridging cell-based and mammalian toxicity assessment. / Webster, Carl A.; Di Silvio, Desire; Devarajan, Aarthi; Bigini, Paolo; Micotti, Edoardo; Giudice, Chiara; Salmona, Mario; Wheeler, Grant N.; Sherwood, Victoria; Bombelli, Francesca Baldelli.

In: Nanomedicine, Vol. 11, No. 6, 01.03.2016, p. 643-656.

Research output: Contribution to journalArticle

Webster, CA, Di Silvio, D, Devarajan, A, Bigini, P, Micotti, E, Giudice, C, Salmona, M, Wheeler, GN, Sherwood, V & Bombelli, FB 2016, 'An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment', Nanomedicine, vol. 11, no. 6, pp. 643-656. https://doi.org/10.2217/nnm.15.219
Webster, Carl A. ; Di Silvio, Desire ; Devarajan, Aarthi ; Bigini, Paolo ; Micotti, Edoardo ; Giudice, Chiara ; Salmona, Mario ; Wheeler, Grant N. ; Sherwood, Victoria ; Bombelli, Francesca Baldelli. / An early developmental vertebrate model for nanomaterial safety : Bridging cell-based and mammalian toxicity assessment. In: Nanomedicine. 2016 ; Vol. 11, No. 6. pp. 643-656.
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