An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells

Lorenzo Spaggiari, Michele Rusca, Paolo Carbognani, Roberta Alfieri, Simonetta Urbani, Leonardo Cattelani, Piergiorgio Petronini, Piergiorgio Solli, Angelo F. Borghetti, Paolo Bobbio

Research output: Contribution to journalArticle

Abstract

Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with those of University of Wisconsin solution (UW) and Euro- Collins solution (EC). Cryopreserved HPAEC tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-plates; subcultures were then incubated at 10°C for 6 hr and 16 hr in 2 ml/well of SPAL UP, UW, and EC. The HPAEC viability after incubation was assessed by evaluating the total protein content and the expression of cytotoxicity, and by analyzing the rate of protein synthesis and expression of cellular functionality after stress. Results after 6 hr of preservation showed that SPAL UP had a less significant cytotoxic effect than EC, exerted a less depressing effect on cellular metabolism, and enhanced functional recovery of endothelial cells compared with UW. At the second time interval (16 hr), SPAL UP provided a less cytotoxic effect than UW; besides, SPAL UP-induced cytotoxicity was similar to that of warm control. In conclusion, in vitro preliminary data regarding the use of SPAL UP in HPAEC preservation suggest its suitability as solution for prolonged lung protection.

Original languageEnglish
Pages (from-to)1369-1371
Number of pages3
JournalTransplantation
Volume62
Issue number9
DOIs
Publication statusPublished - Nov 15 1996

Fingerprint

Pulmonary Artery
Endothelial Cells
Lung
Endothelium
Albumins
Cell Survival
Potassium
Proteins
Cell Culture Techniques
Cell Count
Euro-Collins' solution

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells. / Spaggiari, Lorenzo; Rusca, Michele; Carbognani, Paolo; Alfieri, Roberta; Urbani, Simonetta; Cattelani, Leonardo; Petronini, Piergiorgio; Solli, Piergiorgio; Borghetti, Angelo F.; Bobbio, Paolo.

In: Transplantation, Vol. 62, No. 9, 15.11.1996, p. 1369-1371.

Research output: Contribution to journalArticle

Spaggiari, L, Rusca, M, Carbognani, P, Alfieri, R, Urbani, S, Cattelani, L, Petronini, P, Solli, P, Borghetti, AF & Bobbio, P 1996, 'An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells', Transplantation, vol. 62, no. 9, pp. 1369-1371. https://doi.org/10.1097/00007890-199611150-00036
Spaggiari, Lorenzo ; Rusca, Michele ; Carbognani, Paolo ; Alfieri, Roberta ; Urbani, Simonetta ; Cattelani, Leonardo ; Petronini, Piergiorgio ; Solli, Piergiorgio ; Borghetti, Angelo F. ; Bobbio, Paolo. / An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells. In: Transplantation. 1996 ; Vol. 62, No. 9. pp. 1369-1371.
@article{204faa473cb24813a18ac644ff94dd59,
title = "An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells",
abstract = "Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with those of University of Wisconsin solution (UW) and Euro- Collins solution (EC). Cryopreserved HPAEC tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-plates; subcultures were then incubated at 10°C for 6 hr and 16 hr in 2 ml/well of SPAL UP, UW, and EC. The HPAEC viability after incubation was assessed by evaluating the total protein content and the expression of cytotoxicity, and by analyzing the rate of protein synthesis and expression of cellular functionality after stress. Results after 6 hr of preservation showed that SPAL UP had a less significant cytotoxic effect than EC, exerted a less depressing effect on cellular metabolism, and enhanced functional recovery of endothelial cells compared with UW. At the second time interval (16 hr), SPAL UP provided a less cytotoxic effect than UW; besides, SPAL UP-induced cytotoxicity was similar to that of warm control. In conclusion, in vitro preliminary data regarding the use of SPAL UP in HPAEC preservation suggest its suitability as solution for prolonged lung protection.",
author = "Lorenzo Spaggiari and Michele Rusca and Paolo Carbognani and Roberta Alfieri and Simonetta Urbani and Leonardo Cattelani and Piergiorgio Petronini and Piergiorgio Solli and Borghetti, {Angelo F.} and Paolo Bobbio",
year = "1996",
month = "11",
day = "15",
doi = "10.1097/00007890-199611150-00036",
language = "English",
volume = "62",
pages = "1369--1371",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - An effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells

AU - Spaggiari, Lorenzo

AU - Rusca, Michele

AU - Carbognani, Paolo

AU - Alfieri, Roberta

AU - Urbani, Simonetta

AU - Cattelani, Leonardo

AU - Petronini, Piergiorgio

AU - Solli, Piergiorgio

AU - Borghetti, Angelo F.

AU - Bobbio, Paolo

PY - 1996/11/15

Y1 - 1996/11/15

N2 - Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with those of University of Wisconsin solution (UW) and Euro- Collins solution (EC). Cryopreserved HPAEC tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-plates; subcultures were then incubated at 10°C for 6 hr and 16 hr in 2 ml/well of SPAL UP, UW, and EC. The HPAEC viability after incubation was assessed by evaluating the total protein content and the expression of cytotoxicity, and by analyzing the rate of protein synthesis and expression of cellular functionality after stress. Results after 6 hr of preservation showed that SPAL UP had a less significant cytotoxic effect than EC, exerted a less depressing effect on cellular metabolism, and enhanced functional recovery of endothelial cells compared with UW. At the second time interval (16 hr), SPAL UP provided a less cytotoxic effect than UW; besides, SPAL UP-induced cytotoxicity was similar to that of warm control. In conclusion, in vitro preliminary data regarding the use of SPAL UP in HPAEC preservation suggest its suitability as solution for prolonged lung protection.

AB - Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with those of University of Wisconsin solution (UW) and Euro- Collins solution (EC). Cryopreserved HPAEC tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-plates; subcultures were then incubated at 10°C for 6 hr and 16 hr in 2 ml/well of SPAL UP, UW, and EC. The HPAEC viability after incubation was assessed by evaluating the total protein content and the expression of cytotoxicity, and by analyzing the rate of protein synthesis and expression of cellular functionality after stress. Results after 6 hr of preservation showed that SPAL UP had a less significant cytotoxic effect than EC, exerted a less depressing effect on cellular metabolism, and enhanced functional recovery of endothelial cells compared with UW. At the second time interval (16 hr), SPAL UP provided a less cytotoxic effect than UW; besides, SPAL UP-induced cytotoxicity was similar to that of warm control. In conclusion, in vitro preliminary data regarding the use of SPAL UP in HPAEC preservation suggest its suitability as solution for prolonged lung protection.

UR - http://www.scopus.com/inward/record.url?scp=8044230694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8044230694&partnerID=8YFLogxK

U2 - 10.1097/00007890-199611150-00036

DO - 10.1097/00007890-199611150-00036

M3 - Article

C2 - 8932290

AN - SCOPUS:8044230694

VL - 62

SP - 1369

EP - 1371

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 9

ER -