An efficient method for culturing human breast carcinoma to evaluate antiblastic drug activity in vitro: Experience on 136 primary cancers and on 116 recurrences

Wainer Zoli, Annalisa Volpi, Chiara Bonaguri, Angela Riccobon, Saverio Savini, Rodolfo Brizio, Ariele Saragoni, Laura Medri, Gian Angelo Marra, Dino Amadori

Research output: Contribution to journalArticlepeer-review

Abstract

The feasibility of techniques developed for isolating and culturing human mammary epithelial cells of malignant origin was confirmed in 136 primary breast cancers, 116 hypodermal metastases, and 8 metastatic lymph nodes. In 115 (84%) primary breast cancers and in 81 (70%) hypodermal recurrences we observed a good in vitro cellular proliferation. These proliferating cells, at the second passage, were used for a clonal assay suitable for quantitating drug sensitivity. With this clonal assay median cloning efficiencies of 14% and 6% were obtained respectively in primaries and in skin recurrences. We examined the in vitro response to different drugs and confirmed the test's ability to detect heterogeneity in response to same drugs (doxorubicin, 4′-epidoxorubicin, vinblastine, cis platinum, and idarubicinol) among the different breast carcinoma cultures as well as heterogeneity among subpopulations within a single carcinoma.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalBreast Cancer Research and Treatment
Volume17
Issue number3
DOIs
Publication statusPublished - Jan 1991

Keywords

  • breast cancer cell culture
  • chemosensitivity assay
  • cis platinum
  • doxorubicin
  • drug response
  • epidoxorubicin
  • idarubicinol
  • in vitro
  • vinblastine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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