The molecular technique of polymerase chain reaction (PCR) has been widely used for detection of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL). Gene rearrangements of (T-cell receptor (TCRG) are aspecific markers of leukemic cells in the study of MRD. By the mean of polyacrylamide gel electrophoresis analysis of TCRG-PCR products followed by silver staining (SISTA), we studied 72 children with ALL at onset of disease. This technique allows: - a rapid detection of gene rearrangements avoiding ethidium bromide staining - the identification of heteroduplex bands of clonal nature when both TCRG alleles are rearranged. After SISTA, DNA was recovered from the acrylamide gel, PCR reamplified and directly sequenced. First we identified junctional regions then we constructed patient-specific probes. We obtained a specific probe in 50% of the cases to be used as a molecular marker for MDR.
|Translated title of the contribution||An efficient method of electrophoresis and silver staining for detection of minimal residual disease in acute lymphoblastic leukemia|
|Number of pages||5|
|Publication status||Published - 2001|
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health