An efficient Th2-type memory follows CD8+ lymphocyte-driven and eosinophil-mediated rejection of a spontaneous mouse mammary adenocarcinoma engineered to release IL-4

Federica Pericle, Mirella Giovarelli, Mario P. Colombo, Giuliana Ferrari, Piero Musiani, Andrea Modesti, Federica Cavallo, Francesco Di Pierro, Francesco Novelli, Guido Forni

Research output: Contribution to journalArticlepeer-review

Abstract

A retroviral infection was used to introduce the cDNA coding for mouse IL- 4 into the parental cells of a spontaneous adenocarcinoma of BALB/c mice (TS/A-pc). Four clones releasing between 5 to 40 U of IL-4 (105 cells) in 48 h culture were selected. The secretion of IL-4 does not affect their in vitro growth, whereas their ability to form tumor in vivo inversely correlates with the amount of IL-4 secreted. Although morphologic observation suggested that the rejection of clone D5.40 cells (releasing 40 U of IL-4) depends on eosinophil cytolysis, lymphocyte depletion experiments showed that this required CD8+ lymphocyte guidance. Mice that had rejected D5.40 cells were immune to a subsequent challenge with TS/A-pc. This memory rests on the interaction between noncytotoxic lymphocytes, eosinophils, and IgG1 and IgE anti-TS/A Abs. Comparison of these memory mechanisms with those elicited by IL-2 gene-transduced TS/A cells shows that the kind of cytokine released by the tumor cells determines the type of response. This Th2 memory seems to be more efficient in protecting against a subsequent challenge of TS/A-pc than the Th1-type memory elicited by IL-2 gene-transduced TS/A cells.

Original languageEnglish
Pages (from-to)5659-5673
Number of pages15
JournalJournal of Immunology
Volume153
Issue number12
Publication statusPublished - Dec 15 1994

ASJC Scopus subject areas

  • Immunology

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