TY - JOUR
T1 - An electron microscope study of chromosomal DNA replication in different eukaryotic systems
AU - Micheli, Gioacchino
AU - Baldari, Cosima T.
AU - Carrì, Maria Teresa
AU - Di Cello, Gianni
AU - Buongiorno-Nardelli, Mario
PY - 1982
Y1 - 1982
N2 - We have carried out an electron microscope study of replication intermediates in chromosomal DNA from V. faba root tips; D. melanogaster, X. laevis and chick at different developmental stages; goldfish and brown trout germinal and somatic tissues; regenerating rat liver; Chinese hamster and X. laevis cultured cells. Clusters of small eyes (microbubbles, 100-300 base pairs) and single-stranded DNA molecules have been observed in all the systems analysed, whereas long replication eyes (macrobubbles) were present only in early cleavage embryos of D. melanogaster. The relative frequency of these structures has been compared with similar data from the literature and their relevance as replication intermediates discussed.
AB - We have carried out an electron microscope study of replication intermediates in chromosomal DNA from V. faba root tips; D. melanogaster, X. laevis and chick at different developmental stages; goldfish and brown trout germinal and somatic tissues; regenerating rat liver; Chinese hamster and X. laevis cultured cells. Clusters of small eyes (microbubbles, 100-300 base pairs) and single-stranded DNA molecules have been observed in all the systems analysed, whereas long replication eyes (macrobubbles) were present only in early cleavage embryos of D. melanogaster. The relative frequency of these structures has been compared with similar data from the literature and their relevance as replication intermediates discussed.
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U2 - 10.1016/0014-4827(82)90015-5
DO - 10.1016/0014-4827(82)90015-5
M3 - Article
C2 - 6799307
AN - SCOPUS:0020045372
VL - 137
SP - 127
EP - 140
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -