An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors

Susan M. Huang, Tiziana Bisogno, Marcello Trevisani, Abdulmonem Al-Hayani, Luciano De Petrocellis, Filomena Fezza, Michele Tognetto, Timothy J. Petros, Jocelyn F. Krey, Constance J. Chu, Jeffrey D. Miller, Stephen N. Davies, Pierangelo Geppetti, J. Michael Walker, Vincenzo Di Marzo

Research output: Contribution to journalArticle

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Abstract

The vanilloid receptor VR1 is a nonselective cation channel that is most abundant in peripheral sensory fibers but also is found in several brain nuclei. VR1 is gated by protons, heat, and the pungent ingredient of "hot" chili peppers, capsaicin. To date, no endogenous compound with potency at this receptor comparable to that of capsaicin has been identified. Here we examined the hypothesis, based on previous structure-activity relationship studies and the availability of biosynthetic precursors, that N-arachidonoyl-dopamine (NADA) is an endogenous "capsaicin-like" substance in mammalian nervous tissues. We found that NADA occurs in nervous tissues, with the highest concentrations being found in the striatum, hippocampus, and cerebellum and the lowest concentrations in the dorsal root ganglion. We also gained evidence for the existence of two possible routes for NADA biosynthesis and mechanisms for its inactivation in rat brain. NADA activates both human and rat VR1 overexpressed in human embryonic kidney (HEK)293 cells, with potency (EC50 ≈ 50 nM) and efficacy similar to those of capsaicin. Furthermore, NADA potently activates native vanilloid receptors in neurons from rat dorsal root ganglion and hippocampus, thereby inducing the release of substance P and calcitonin gene-related peptide (CGRP) from dorsal spinal cord slices and enhancing hippocampal paired-pulse depression, respectively. Intradermal NADA also induces VR1-mediated thermal hyperalgesia (EC50 = 1.5 ± 0.3 μg). Our data demonstrate the existence of a brain substance similar to capsaicin not only with respect to its chemical structure but also to its potency at VR1 receptors.

Original languageEnglish
Pages (from-to)8400-8405
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number12
DOIs
Publication statusPublished - Jun 11 2002

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Capsaicin
Dopamine
Nerve Tissue
Capsicum
Spinal Ganglia
Hippocampus
Brain
TRPV Cation Channels
Calcitonin Gene-Related Peptide
Hyperalgesia
Structure-Activity Relationship
Substance P
Cerebellum
TRPV1 receptor
Cations
Protons
Spinal Cord
Hot Temperature
Kidney
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. / Huang, Susan M.; Bisogno, Tiziana; Trevisani, Marcello; Al-Hayani, Abdulmonem; De Petrocellis, Luciano; Fezza, Filomena; Tognetto, Michele; Petros, Timothy J.; Krey, Jocelyn F.; Chu, Constance J.; Miller, Jeffrey D.; Davies, Stephen N.; Geppetti, Pierangelo; Walker, J. Michael; Di Marzo, Vincenzo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 12, 11.06.2002, p. 8400-8405.

Research output: Contribution to journalArticle

Huang, SM, Bisogno, T, Trevisani, M, Al-Hayani, A, De Petrocellis, L, Fezza, F, Tognetto, M, Petros, TJ, Krey, JF, Chu, CJ, Miller, JD, Davies, SN, Geppetti, P, Walker, JM & Di Marzo, V 2002, 'An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 12, pp. 8400-8405. https://doi.org/10.1073/pnas.122196999
Huang, Susan M. ; Bisogno, Tiziana ; Trevisani, Marcello ; Al-Hayani, Abdulmonem ; De Petrocellis, Luciano ; Fezza, Filomena ; Tognetto, Michele ; Petros, Timothy J. ; Krey, Jocelyn F. ; Chu, Constance J. ; Miller, Jeffrey D. ; Davies, Stephen N. ; Geppetti, Pierangelo ; Walker, J. Michael ; Di Marzo, Vincenzo. / An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 12. pp. 8400-8405.
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AU - De Petrocellis, Luciano

AU - Fezza, Filomena

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AU - Petros, Timothy J.

AU - Krey, Jocelyn F.

AU - Chu, Constance J.

AU - Miller, Jeffrey D.

AU - Davies, Stephen N.

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