An Engineered Cell-Instructive Stroma for the Fabrication of a Novel Full Thickness Human Cervix Equivalent In Vitro

Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo, Maria L Tornesello, Clorinda Annunziata, Franco M Buonaguro, Paolo Antonio Netti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

There is a growing interest for developing organotypic cervical models by using primary cervical cells that are able to reproduce the physiological relevant stromal microenvironment and the distinctive histology of the native cervical epithelium. Here for the first time it is reported the production of an organotypic cervical model featured by a scaffold-free stromal tissue resembling the extracellular matrix (ECM) composition and organization of the native counterpart as well as a completely well-differentiated epithelium. To reach this aim, human cervical microtissue precursors have been produced, characterized, and used as functional building units to fabricate a cell-synthesized cervical stroma equivalent by means of a bottom-up approach. Immunotypization, and molecular and morphological analyses reveal the extent of fundamental epithelial biomarkers and the presence of collagen and noncollagenous molecules, demonstrating that the natural tissue architecture and biological characteristics of cervical tissues are reproduced. The results of this study suggest that the bottom-up technology used to produce these 3D human cervical equivalents provides a fully functional organotypic cervical model that may be used as a valuable tool to investigate the epithelial-stromal interactions as well as for testing new therapeutics in vitro.

Original languageEnglish
JournalAdvanced healthcare materials
DOIs
Publication statusPublished - Jun 2017

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Cervix Uteri
Tissue
Fabrication
Epithelium
Histology
Biomarkers
Collagen
Scaffolds
Extracellular Matrix
Technology
Molecules
Testing
Chemical analysis
In Vitro Techniques
Therapeutics

Keywords

  • Journal Article

Cite this

An Engineered Cell-Instructive Stroma for the Fabrication of a Novel Full Thickness Human Cervix Equivalent In Vitro. / De Gregorio, Vincenza; Imparato, Giorgia; Urciuolo, Francesco; Tornesello, Maria L; Annunziata, Clorinda; Buonaguro, Franco M; Netti, Paolo Antonio.

In: Advanced healthcare materials, 06.2017.

Research output: Contribution to journalArticle

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AU - Urciuolo, Francesco

AU - Tornesello, Maria L

AU - Annunziata, Clorinda

AU - Buonaguro, Franco M

AU - Netti, Paolo Antonio

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