An Enzyme-linked Immunosorbent Assay for the Direct Analysis of Beta-agonist Drugs in Urine and Sera

M. Paleologo Oriundi, G. Giacomini Giacomini, F. Ballaben, E. B. Bastiani, F. Berti, F. Benedetti, R. Bagnati

Research output: Contribution to journalArticle

Abstract

A sensitive competitive solid-phase enzyme immunoassay for the detection of tert-butylic beta-agonist drugs was developed. An antiserum was raised in rabbits against the diazoconjugate of clenbuterol and the human serum albumin. In the assay, the anticlenbuterol antibody was incubated with the salbutamol-enzyme conjugate and unlabelled standard or samples in microtitre wells precoated with affinity-purified antirabbit IgG. The absolute detection limit for clenbuterol and mabuterol was 0.5 ng/ml (15 pg/well) and 50% relative binding at 1.0 ng/ml (50 pg/well). The absolute detection limits for salbutamol and terbutaline were 0.9 and 1.3 ng/ml respectively. The cross-reactivity of iso-propylic parent compounds was quite low (

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalFood and Agricultural Immunology
Volume4
Issue number2
DOIs
Publication statusPublished - 1992

Keywords

  • anabolic agents
  • beta-agonists
  • ELISA

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Immunology

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