An ex-vivo model of shear-rate-based activation of blood coagulation

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Abstract

The study presents a model of shear-stress-based platelet activation. Twenty-eight patients (22 free from anticoagulants and major antiplatelet agents, and six under the effects of P2Y12 platelet inhibitors) participated. The main purpose was to verify the hypothesis that a model of shear-dependent blood activation does not require artificial activators to trigger clot formation. Whole blood collected from the patients received platelet function tests [ADPtest and thrombin receptor-Activating peptide (TRAP)test] and was tested with a cone-on-plate viscosimeter at a shear rate of 100 sS-1. Changes in blood viscosity were characterized by a time-To-gel point (TGP), a maximum clot viscosity and a steady clot viscosity (SCV). In patients free from major antiplatelet effects, the TGP was 180 s (interquartile range 148-290 s), while in patients under double antiplatelet therapy the TGP was significantly (P=0.039) longer (345 s, interquartile range 250-452 s). The SCV was 16 centipoise (cP) (interquartile range 11-47 cP) in the patients free from major antiplatelet agents, significantly (P=0.012) higher than in patients under double antiplatelet therapy (10 cP, interquartile range 6-11 cP). There was a significant (P=0.011) association between platelet function at the TRAPtest and the maximum clot viscosity, and between TRAPtest and the SCV (P=0.021). A shear rate of 100 sS1 triggers clot formation through a primary role of platelet activation in this model of blood activation. Blood Coagul Fibrinolysis 29:172-177

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume29
Issue number2
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Blood Coagulation
Viscosity
Platelet Aggregation Inhibitors
thrombin receptor peptide SFLLRNP
Gels
Platelet Activation
Platelet Function Tests
Blood Viscosity
Fibrinolysis
Anticoagulants
Blood Platelets
Therapeutics

Keywords

  • blood coagulation
  • blood viscosity
  • platelets

ASJC Scopus subject areas

  • Hematology

Cite this

An ex-vivo model of shear-rate-based activation of blood coagulation. / Ranucci, Marco; Ranucci, Matteo; Baryshnikova, Ekaterina.

In: Blood Coagulation and Fibrinolysis, Vol. 29, No. 2, 01.03.2018, p. 172-177.

Research output: Contribution to journalArticle

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