An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures

G. Regalia, E. Biffi, A. Lucchini, M. Capriata, S. Achilli, A. Menegon, G. Ferrigno, L. Colombo, A. Pedrocchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The elucidation of physio-pathological mechanisms expressed by a neuronal network over extended time scales (i.e., from hours to weeks) is the goal of many neurophysiological and neuropharmacological in vitro studies. In this context, a challenging technological requirement is the establishment of an experimental setup able to collect long-term neuronal signals. In this work we report the development of a compact environmental chamber designed to perform prolonged recordings of the bioelectrical activity exhibited by neuronal networks grown on MicroElectrode Arrays (MEAs). To reproduce an environment suitable for cells growth (temperature, pH and humidity) the chamber was coupled with a temperature control system and an air humidifying module. Validation tests demonstrated that the environment inside the portable chamber is comparable to standard cell incubators environment. To collect neuronal extracellular signals, custom multichannel pre-processing boards have been developed and integrated with the chamber. With this equipment, we were able to reliably record spontaneous neuronal electrical activity from hippocampal cultures grown inside the chamber for several hours, which is not possible with the standard MEA recording setup due to environmental fluctuations. This system can collect multichannel data from neuronal cultures over long periods, providing an effective solution for long-term studies of neural activity.

Original languageEnglish
Title of host publicationIJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence
Pages551-557
Number of pages7
Publication statusPublished - 2013
Event5th International Joint Conference on Computational Intelligence, IJCCI 2013 - Vilamoura, Algarve, Portugal
Duration: Sep 20 2013Sep 22 2013

Other

Other5th International Joint Conference on Computational Intelligence, IJCCI 2013
CountryPortugal
CityVilamoura, Algarve
Period9/20/139/22/13

Fingerprint

Microelectrodes
Environmental chambers
Cell growth
Growth temperature
Temperature control
Atmospheric humidity
Control systems
Processing
Air

Keywords

  • Long-term recordings
  • Microelectrode arrays
  • Neuronal cultures

ASJC Scopus subject areas

  • Software

Cite this

Regalia, G., Biffi, E., Lucchini, A., Capriata, M., Achilli, S., Menegon, A., ... Pedrocchi, A. (2013). An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures. In IJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence (pp. 551-557)

An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures. / Regalia, G.; Biffi, E.; Lucchini, A.; Capriata, M.; Achilli, S.; Menegon, A.; Ferrigno, G.; Colombo, L.; Pedrocchi, A.

IJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence. 2013. p. 551-557.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Regalia, G, Biffi, E, Lucchini, A, Capriata, M, Achilli, S, Menegon, A, Ferrigno, G, Colombo, L & Pedrocchi, A 2013, An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures. in IJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence. pp. 551-557, 5th International Joint Conference on Computational Intelligence, IJCCI 2013, Vilamoura, Algarve, Portugal, 9/20/13.
Regalia G, Biffi E, Lucchini A, Capriata M, Achilli S, Menegon A et al. An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures. In IJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence. 2013. p. 551-557
Regalia, G. ; Biffi, E. ; Lucchini, A. ; Capriata, M. ; Achilli, S. ; Menegon, A. ; Ferrigno, G. ; Colombo, L. ; Pedrocchi, A. / An experimental platform aimed at long lasting electrophysiological multichannel recordings of neuronal cultures. IJCCI 2013 - Proceedings of the 5th International Joint Conference on Computational Intelligence. 2013. pp. 551-557
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