An experimental study in the chick embryo chorioallantoic membrane of the anti-angiogenic activity of cyclosporine in rheumatoid arthritis versus osteoarthritis

D. Ribatti, A. Vacca, F. P. Cantatore, R. Ria, V. Benagiano, L. Roncali, F. Dammacco

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective and Design. Angiogenesis plays an important role in the pathogenesis of rheumatoid arthritis (RA) and correlates with clinical score, synovial hyperplasia and infiltration of inflammatory cells. Many of the available treatments for RA have been shown to possess some degree of anti-angiogenic activity. Here, we studied the effect of cyclosporine, which exerts anti-angiogenic activity in vitro and in vivo [1] on angiogenesis induced in vivo in the chick embryo chorioallantoic membrane (CAM) by synovial RA and osteoarthritis (OA) tissues. Material and Methods. Wet synovial biopsies from 10 RA and 6 OA patients were treated with vehicle alone or with cyclosporine and implanted on the CAM at day 8 of incubation. On day 12, CAM tissues were assessed for the extent of angiogenesis and mononuclear cell infiltration. Results. Cyclosporine inhibited angiogenesis and reduced the number of mononuclear cells in the CAM extracellular matrix only in RA implants. Conclusions. These data provide further evidence for a central role of new-formed blood vessels in RA. Moreover, cyclosporine on account of both its immunosuppressive and its anti-angiogenic activity can be proposed for the treatment of RA.

Original languageEnglish
Pages (from-to)418-423
Number of pages6
JournalInflammation Research
Volume49
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint

Chorioallantoic Membrane
Chick Embryo
Osteoarthritis
Cyclosporine
Rheumatoid Arthritis
Membranes
Infiltration
Tissue
Biopsy
Blood vessels
Immunosuppressive Agents
Hyperplasia
Extracellular Matrix
Blood Vessels
Cell Count
Therapeutics

Keywords

  • Anti-angiogenesis
  • Chorioallantoic membrane
  • Cyclosporine
  • Osteoarthritis
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

An experimental study in the chick embryo chorioallantoic membrane of the anti-angiogenic activity of cyclosporine in rheumatoid arthritis versus osteoarthritis. / Ribatti, D.; Vacca, A.; Cantatore, F. P.; Ria, R.; Benagiano, V.; Roncali, L.; Dammacco, F.

In: Inflammation Research, Vol. 49, No. 8, 2000, p. 418-423.

Research output: Contribution to journalArticle

Ribatti, D. ; Vacca, A. ; Cantatore, F. P. ; Ria, R. ; Benagiano, V. ; Roncali, L. ; Dammacco, F. / An experimental study in the chick embryo chorioallantoic membrane of the anti-angiogenic activity of cyclosporine in rheumatoid arthritis versus osteoarthritis. In: Inflammation Research. 2000 ; Vol. 49, No. 8. pp. 418-423.
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