An improved electrophoretic method for separating and quantifying bone and liver alkaline phosphatase isoenzymes in serum

S. Secchiero, R. Bertorelle, M. Zaninotto, A. Burlina

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We describe a new, simple and rapid method for the separation and quantification of bone and liver isoenzymes of alkaline phosphatase (EC 3.1.3.1) in serum. We have utilized a lectin and neuraminidase mixture put together with the serum patient, directly in the well, and incubated for 10 minutes, before electrophoresis on cellulose acetate. So, in the same plate it is possible to compare untreated and treated samples without a further electrophoretic run and which other methods need for identificantion of isoenzymes different from bone and liver. Utilizing our procedure, the bone isoenzyme is clearly separated from the liver fraction allowing these isoenzymes to be quantified by densitometry.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalClinical Chemistry and Enzymology Communications
Volume1
Issue number3
Publication statusPublished - 1989

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Liver
Isoenzymes
Alkaline Phosphatase
Bone
Bone and Bones
Serum
Cellulose Acetate Electrophoresis
Densitometry
Neuraminidase
Electrophoresis
Lectins

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

An improved electrophoretic method for separating and quantifying bone and liver alkaline phosphatase isoenzymes in serum. / Secchiero, S.; Bertorelle, R.; Zaninotto, M.; Burlina, A.

In: Clinical Chemistry and Enzymology Communications, Vol. 1, No. 3, 1989, p. 185-192.

Research output: Contribution to journalArticle

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