An improved method for increasing the sensitivity of direct agglutination test (D.A.T.) to the detection of IgM and IgG anti-toxoplasma antibodies.

G. Filice, G. Carnevale, V. Meroni, V. Gagliardi, F. Castelli, G. Carosi

Research output: Contribution to journalArticle

Abstract

The present investigation has shown that the antibody titers obtained by IgG-IFA correlate well with 2-ME-DA (correlation coefficient 0.927, p less than 0.001). These results are obtained when the antibody titers are expressed as IU/ml. Buffer without 2-ME and purification of IgM fractions by simple gel-filtration method seem to be necessary conditions for detecting IgM antibodies by DA test in adult patients. Therefore the DA with these micromodifications has a specificity and sensitivity similar to those of IgG and IgM-IFA tests and would be convenient for laboratories that perform large scale surveys during pregnancy.

Original languageEnglish
Pages (from-to)458-461
Number of pages4
JournalBollettino dell'Istituto Sieroterapico Milanese
Volume63
Issue number5
Publication statusPublished - 1984

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Agglutination Tests
Toxoplasma
Immunoglobulin M
Anti-Idiotypic Antibodies
Mercaptoethanol
Immunoglobulin G
Antibodies
Gel Chromatography
Buffers
Sensitivity and Specificity
Pregnancy

ASJC Scopus subject areas

  • Immunology

Cite this

An improved method for increasing the sensitivity of direct agglutination test (D.A.T.) to the detection of IgM and IgG anti-toxoplasma antibodies. / Filice, G.; Carnevale, G.; Meroni, V.; Gagliardi, V.; Castelli, F.; Carosi, G.

In: Bollettino dell'Istituto Sieroterapico Milanese, Vol. 63, No. 5, 1984, p. 458-461.

Research output: Contribution to journalArticle

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AU - Gagliardi, V.

AU - Castelli, F.

AU - Carosi, G.

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