An improved method for liquid scintillation counting of 59Fe in ferrokinetic studies

S. Perugini, G. Barosi, M. Cazzola

Research output: Contribution to journalArticle

Abstract

An improved method for liquid scintillation counting of 59Fe in plasma and whole blood is described. About 2 ml of venous heparinized blood are required for the assay of the radioiron in plasma and erythrocytes. Soluene 100 as a solubilizer and Instagel as a scintillation solution are used in sample preparation. In ferrokinetic studies this method has 3 advantages over the conventional method for 59Fe gamma counting: it allows a reduction in the total blood withdrawal from 120-150 to about 40 ml and in the total counting time from 4-5 to about 2 days, with a 4 or 5 fold increase in counting efficiency. The high counting efficiency and the low background allow better counting statistics, particularly for the low activity plasma samples from the 2nd to the 14th day of study. Both in normal volunteers and in anemic patients this method has revealed transient elevations in plasma radioactivity, which cannot easily be detected by conventional gamma counting of 59Fe.

Original languageEnglish
Pages (from-to)54-67
Number of pages14
JournalHaematologica
Volume59
Issue number1
Publication statusPublished - 1974

ASJC Scopus subject areas

  • Hematology

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