An improved PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in formalin-fixed stools

Maria Grazia Paglia, Paolo Visca

Research output: Contribution to journalArticle

Abstract

Detection of infectious agents in faecal samples by polymerase chain reaction (PCR) can be limited by the presence of substances which inhibit DNA amplification. Here, an improved protocol is reported for directly isolating DNA from fresh and aged formalin-fixed stools, after concentration by formalin-ethyl acetate (FEA). The protocol was successfully applied to detect DNA of Entamoeba histolytica/Entamoeba dispar complex in stools by nested PCR, showing high specificity and low detection limit. Extended time of specimen storage in formalin had no influence on PCR yields. This PCR-based method offers technical advantages for routine detection and discrimination of invasive E. histolytica and non-invasive E. dispar.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalActa Tropica
Volume92
Issue number3
DOIs
Publication statusPublished - Nov 2004

Keywords

  • Entamoeba coli
  • Entamoeba dispar
  • Entamoeba histolytica

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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