An improved procedure for in situ RTPCR

L. Volpi, L. Doneda, M. G. Grimoldi, E. Pollina, L. Larizza

Research output: Contribution to journal › Article

Abstract

Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalMolecular and Cellular Probes
Volume10
Issue number1
DOIs
Publication statusPublished - Feb 1996

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Stem Cell Factor
Trophoblasts
Stromal Cells
Paraffin
Reverse Transcription
Messenger RNA

Keywords

  • Immunohistochemical detection
  • Paraffin embedded tissue sections
  • RTPCR in situ

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

An improved procedure for in situ RTPCR. / Volpi, L.; Doneda, L.; Grimoldi, M. G.; Pollina, E.; Larizza, L.

In: Molecular and Cellular Probes, Vol. 10, No. 1, 02.1996, p. 63-66.

Research output: Contribution to journal › Article

Volpi, L, Doneda, L, Grimoldi, MG, Pollina, E & Larizza, L 1996, 'An improved procedure for in situ RTPCR', Molecular and Cellular Probes, vol. 10, no. 1, pp. 63-66. https://doi.org/10.1006/mcpr.1996.0009
Volpi L, Doneda L, Grimoldi MG, Pollina E, Larizza L. An improved procedure for in situ RTPCR. Molecular and Cellular Probes. 1996 Feb;10(1):63-66. https://doi.org/10.1006/mcpr.1996.0009
Volpi, L. ; Doneda, L. ; Grimoldi, M. G. ; Pollina, E. ; Larizza, L. / An improved procedure for in situ RTPCR. In: Molecular and Cellular Probes. 1996 ; Vol. 10, No. 1. pp. 63-66.
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