An improved procedure for in situ RTPCR

L. Volpi, L. Doneda, M. G. Grimoldi, E. Pollina, L. Larizza

Research output: Contribution to journalArticlepeer-review

Abstract

Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalMolecular and Cellular Probes
Volume10
Issue number1
DOIs
Publication statusPublished - Feb 1996

Keywords

  • Immunohistochemical detection
  • Paraffin embedded tissue sections
  • RTPCR in situ

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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