An improved procedure for in situ RTPCR

L. Volpi; L. Doneda; M. G. Grimoldi; E. Pollina; L. Larizza

doi:10.1006/mcpr.1996.0009

An improved procedure for in situ RTPCR

L. Volpi, L. Doneda, M. G. Grimoldi, E. Pollina, L. Larizza

Fondazione Istituto Auxologico Italiano

Research output: Contribution to journal › Article › peer-review

Abstract

Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

Original language	English
Pages (from-to)	63-66
Number of pages	4
Journal	Molecular and Cellular Probes
Volume	10
Issue number	1
DOIs	https://doi.org/10.1006/mcpr.1996.0009
Publication status	Published - Feb 1996

Keywords

Immunohistochemical detection
Paraffin embedded tissue sections
RTPCR in situ

ASJC Scopus subject areas

Cell Biology
Molecular Biology

Access to Document

10.1006/mcpr.1996.0009

Cite this

@article{4e5471fa20894ca5a489855ad26cf797,

title = "An improved procedure for in situ RTPCR",

abstract = "Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.",

keywords = "Immunohistochemical detection, Paraffin embedded tissue sections, RTPCR in situ",

author = "L. Volpi and L. Doneda and Grimoldi, {M. G.} and E. Pollina and L. Larizza",

year = "1996",

month = feb,

doi = "10.1006/mcpr.1996.0009",

language = "English",

volume = "10",

pages = "63--66",

journal = "Molecular and Cellular Probes",

issn = "0890-8508",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - An improved procedure for in situ RTPCR

AU - Volpi, L.

AU - Doneda, L.

AU - Grimoldi, M. G.

AU - Pollina, E.

AU - Larizza, L.

PY - 1996/2

Y1 - 1996/2

N2 - Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

AB - Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

KW - Immunohistochemical detection

KW - Paraffin embedded tissue sections

KW - RTPCR in situ

UR - http://www.scopus.com/inward/record.url?scp=0029923247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029923247&partnerID=8YFLogxK

U2 - 10.1006/mcpr.1996.0009

DO - 10.1006/mcpr.1996.0009

M3 - Article

C2 - 8684378

AN - SCOPUS:0029923247

VL - 10

SP - 63

EP - 66

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

IS - 1

ER -

An improved procedure for in situ RTPCR

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this