Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.
- Immunohistochemical detection
- Paraffin embedded tissue sections
- RTPCR in situ
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology