An improved procedure for in situ RTPCR

L. Volpi; L. Doneda; M. G. Grimoldi; E. Pollina; L. Larizza

doi:10.1006/mcpr.1996.0009

An improved procedure for in situ RTPCR

L. Volpi, L. Doneda, M. G. Grimoldi, E. Pollina, L. Larizza

Fondazione Istituto Auxologico Italiano

Research output: Contribution to journal › Article › peer-review

Abstract

Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.

Original language	English
Pages (from-to)	63-66
Number of pages	4
Journal	Molecular and Cellular Probes
Volume	10
Issue number	1
DOIs	https://doi.org/10.1006/mcpr.1996.0009
Publication status	Published - Feb 1996

Keywords

Immunohistochemical detection
Paraffin embedded tissue sections
RTPCR in situ

ASJC Scopus subject areas

Cell Biology
Molecular Biology

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10.1006/mcpr.1996.0009

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title = "An improved procedure for in situ RTPCR",

abstract = "Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.",

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AU - Grimoldi, M. G.

AU - Pollina, E.

AU - Larizza, L.

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KW - RTPCR in situ

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