An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis

Manuela Grassi, Elisabetta Volpe, Vittorio Colizzi, Francesca Mariani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalJournal of Microbiological Methods
Volume64
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Mycobacterium tuberculosis
Real-Time Polymerase Chain Reaction
RNA
Sensitivity and Specificity
Polymerase Chain Reaction
Messenger RNA
formamide

Keywords

  • Co-solvents
  • GC rich transcripts
  • Intracellular pathogen
  • Quantitation
  • Specificity

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis. / Grassi, Manuela; Volpe, Elisabetta; Colizzi, Vittorio; Mariani, Francesca.

In: Journal of Microbiological Methods, Vol. 64, No. 3, 03.2006, p. 406-410.

Research output: Contribution to journalArticle

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