The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.
- GC rich transcripts
- Intracellular pathogen
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology