An improved RT-PCR protocol for the quantitation of human retinoic acid receptor RNA

N. Ferrari, U. Pfeffer, F. Tosetti, C. Brigati, G. Vidali

Research output: Contribution to journalArticle

Abstract

A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) system has been developed to calculate the level of expression of human retinoic acid receptors (hRAR) α, β, and γ. Starting from a single cDNA preparation, the system allows the measurement of the number of molecules of each mRNA receptor. This is made possible by a synthetic internal standard mRNA which is added in known concentrations at the beginning of the reaction. The system is tested in a rhabdomyosarcoma cell line (A-673) where we have measured the upregulation of β and γ receptor mRNAs following treatment with retinoic acid.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalExperimental Cell Research
Volume211
Issue number1
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'An improved RT-PCR protocol for the quantitation of human retinoic acid receptor RNA'. Together they form a unique fingerprint.

  • Cite this