An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells.

Francesca Salamanna, Veronica Borsari, Silvia Brogini, Gianluca Giavaresi, Annapaola Parrilli, Simona Cepollaro, Matteo Cadossi, Lucia Martini, A. Mazzotti, Milena Fini, Milena Fini

Research output: Contribution to journalArticle

Abstract

One of the main limitations, when studying cancer-bone metastasis, is the complex nature of the native bone environment and the lack of reliable, simple, inexpensive models that closely mimic the biological processes occurring in patients and allowing the correct translation of results. To enhance the understanding of the mechanisms underlying human bone metastases and in order to find new therapies, we developed an in vitro three-dimensional (3D) cancer-bone metastasis model by culturing human breast or prostate cancer cells with human bone tissue isolated from female and male patients, respectively. Bone tissue discarded from total hip replacement surgery was cultured in a rolling apparatus system in a normoxic or hypoxic environment. Gene expression profile, protein levels, histological, immunohistochemical and four-dimensional (4D) micro-CT analyses showed a noticeable specificity of breast and prostate cancer cells for bone colonization and ingrowth, thus highlighting the species-specific and sex-specific osteotropism and the need to widen the current knowledge on cancer-bone metastasis spread in human bone tissues. The results of this study support the application of this model in preclinical studies on bone metastases and also follow the 3R principles, the guiding principles, aimed at replacing/reducing/refining (3R) animal use and their suffering for scientific purposes.

Original languageEnglish
Pages (from-to)76966-76983
Number of pages18
JournalOncotarget
Volume7
Issue number47
DOIs
Publication statusPublished - Oct 19 2016

Fingerprint

Bone Neoplasms
Neoplasm Metastasis
Bone and Bones
Neoplasms
Prostatic Neoplasms
Four-Dimensional Computed Tomography
Breast Neoplasms
Biological Phenomena
Hip Replacement Arthroplasties
Transcriptome
Psychological Stress
In Vitro Techniques
Proteins
Therapeutics

Cite this

An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells. / Salamanna, Francesca; Borsari, Veronica; Brogini, Silvia; Giavaresi, Gianluca; Parrilli, Annapaola; Cepollaro, Simona; Cadossi, Matteo; Martini, Lucia; Mazzotti, A.; Fini, Milena; Fini, Milena.

In: Oncotarget, Vol. 7, No. 47, 19.10.2016, p. 76966-76983.

Research output: Contribution to journalArticle

Salamanna, Francesca ; Borsari, Veronica ; Brogini, Silvia ; Giavaresi, Gianluca ; Parrilli, Annapaola ; Cepollaro, Simona ; Cadossi, Matteo ; Martini, Lucia ; Mazzotti, A. ; Fini, Milena ; Fini, Milena. / An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells. In: Oncotarget. 2016 ; Vol. 7, No. 47. pp. 76966-76983.
@article{adbd2fd2240b4bd18b5976125cce6368,
title = "An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells.",
abstract = "One of the main limitations, when studying cancer-bone metastasis, is the complex nature of the native bone environment and the lack of reliable, simple, inexpensive models that closely mimic the biological processes occurring in patients and allowing the correct translation of results. To enhance the understanding of the mechanisms underlying human bone metastases and in order to find new therapies, we developed an in vitro three-dimensional (3D) cancer-bone metastasis model by culturing human breast or prostate cancer cells with human bone tissue isolated from female and male patients, respectively. Bone tissue discarded from total hip replacement surgery was cultured in a rolling apparatus system in a normoxic or hypoxic environment. Gene expression profile, protein levels, histological, immunohistochemical and four-dimensional (4D) micro-CT analyses showed a noticeable specificity of breast and prostate cancer cells for bone colonization and ingrowth, thus highlighting the species-specific and sex-specific osteotropism and the need to widen the current knowledge on cancer-bone metastasis spread in human bone tissues. The results of this study support the application of this model in preclinical studies on bone metastases and also follow the 3R principles, the guiding principles, aimed at replacing/reducing/refining (3R) animal use and their suffering for scientific purposes.",
author = "Francesca Salamanna and Veronica Borsari and Silvia Brogini and Gianluca Giavaresi and Annapaola Parrilli and Simona Cepollaro and Matteo Cadossi and Lucia Martini and A. Mazzotti and Milena Fini and Milena Fini",
year = "2016",
month = "10",
day = "19",
doi = "10.18632/oncotarget.12763",
language = "English",
volume = "7",
pages = "76966--76983",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "47",

}

TY - JOUR

T1 - An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells.

AU - Salamanna, Francesca

AU - Borsari, Veronica

AU - Brogini, Silvia

AU - Giavaresi, Gianluca

AU - Parrilli, Annapaola

AU - Cepollaro, Simona

AU - Cadossi, Matteo

AU - Martini, Lucia

AU - Mazzotti, A.

AU - Fini, Milena

AU - Fini, Milena

PY - 2016/10/19

Y1 - 2016/10/19

N2 - One of the main limitations, when studying cancer-bone metastasis, is the complex nature of the native bone environment and the lack of reliable, simple, inexpensive models that closely mimic the biological processes occurring in patients and allowing the correct translation of results. To enhance the understanding of the mechanisms underlying human bone metastases and in order to find new therapies, we developed an in vitro three-dimensional (3D) cancer-bone metastasis model by culturing human breast or prostate cancer cells with human bone tissue isolated from female and male patients, respectively. Bone tissue discarded from total hip replacement surgery was cultured in a rolling apparatus system in a normoxic or hypoxic environment. Gene expression profile, protein levels, histological, immunohistochemical and four-dimensional (4D) micro-CT analyses showed a noticeable specificity of breast and prostate cancer cells for bone colonization and ingrowth, thus highlighting the species-specific and sex-specific osteotropism and the need to widen the current knowledge on cancer-bone metastasis spread in human bone tissues. The results of this study support the application of this model in preclinical studies on bone metastases and also follow the 3R principles, the guiding principles, aimed at replacing/reducing/refining (3R) animal use and their suffering for scientific purposes.

AB - One of the main limitations, when studying cancer-bone metastasis, is the complex nature of the native bone environment and the lack of reliable, simple, inexpensive models that closely mimic the biological processes occurring in patients and allowing the correct translation of results. To enhance the understanding of the mechanisms underlying human bone metastases and in order to find new therapies, we developed an in vitro three-dimensional (3D) cancer-bone metastasis model by culturing human breast or prostate cancer cells with human bone tissue isolated from female and male patients, respectively. Bone tissue discarded from total hip replacement surgery was cultured in a rolling apparatus system in a normoxic or hypoxic environment. Gene expression profile, protein levels, histological, immunohistochemical and four-dimensional (4D) micro-CT analyses showed a noticeable specificity of breast and prostate cancer cells for bone colonization and ingrowth, thus highlighting the species-specific and sex-specific osteotropism and the need to widen the current knowledge on cancer-bone metastasis spread in human bone tissues. The results of this study support the application of this model in preclinical studies on bone metastases and also follow the 3R principles, the guiding principles, aimed at replacing/reducing/refining (3R) animal use and their suffering for scientific purposes.

U2 - 10.18632/oncotarget.12763

DO - 10.18632/oncotarget.12763

M3 - Article

VL - 7

SP - 76966

EP - 76983

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 47

ER -