TY - JOUR
T1 - An in vivo autotransplant model of renal preservation
T2 - Cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury
AU - La Manna, Gaetano
AU - Conte, Diletta
AU - Cappuccilli, Maria Laura
AU - Nardo, Bruno
AU - D'Addio, Francesca
AU - Puviani, Lorenza
AU - Comai, Giorgia
AU - Bianchi, Francesca
AU - Bertelli, Riccardo
AU - Lanci, Nicole
AU - Donati, Gabriele
AU - Scolari, Maria Piera
AU - Faenza, Alessandro
AU - Stefoni, Sergio
PY - 2009/7
Y1 - 2009/7
N2 - There is increasing proof that organ preservation by machine perfusion is able to limit ischemia/reperfusion injury in kidney transplantation. This study was designed to compare the efficiency in hypothermic organ preservation by machine perfusion or cold storage in an animal model of kidney autotransplantation. Twelve pigs underwent left nephrectomy after warm ischemic time; the organs were preserved in machine perfusion (n = 6) or cold storage (n = 6) and then autotransplanted with immediate contralateral nephrectomy. The following parameters were compared between the two groups of animals: hematological and urine indexes of renal function, blood/gas analysis values, histological features, tissue adenosine-5′-triphosphate (ATP) content, perforin gene expression in kidney biopsies, and organ weight changes were compared before and after preservation. The amount of cellular ATP was significantly higher in organs preserved by machine perfusion; moreover, the study of apoptosis induction revealed an enhanced perforin expression in the kidneys, which underwent simple hypothermic preservation compared to the machine-preserved ones. Organ weight was significantly decreased after cold storage, but it remained quite stable for machine-perfused kidneys. The present model seems to suggest that organ preservation by hypothermic machine perfusion is able to better control cellular impairment in comparison with cold storage.
AB - There is increasing proof that organ preservation by machine perfusion is able to limit ischemia/reperfusion injury in kidney transplantation. This study was designed to compare the efficiency in hypothermic organ preservation by machine perfusion or cold storage in an animal model of kidney autotransplantation. Twelve pigs underwent left nephrectomy after warm ischemic time; the organs were preserved in machine perfusion (n = 6) or cold storage (n = 6) and then autotransplanted with immediate contralateral nephrectomy. The following parameters were compared between the two groups of animals: hematological and urine indexes of renal function, blood/gas analysis values, histological features, tissue adenosine-5′-triphosphate (ATP) content, perforin gene expression in kidney biopsies, and organ weight changes were compared before and after preservation. The amount of cellular ATP was significantly higher in organs preserved by machine perfusion; moreover, the study of apoptosis induction revealed an enhanced perforin expression in the kidneys, which underwent simple hypothermic preservation compared to the machine-preserved ones. Organ weight was significantly decreased after cold storage, but it remained quite stable for machine-perfused kidneys. The present model seems to suggest that organ preservation by hypothermic machine perfusion is able to better control cellular impairment in comparison with cold storage.
KW - Delayed graft function
KW - Ischemia/reperfusion injury
KW - Kidney function
KW - Machine perfusion
KW - Organ preservation
KW - Renal transplantation
UR - http://www.scopus.com/inward/record.url?scp=67650799641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650799641&partnerID=8YFLogxK
U2 - 10.1111/j.1525-1594.2009.00743.x
DO - 10.1111/j.1525-1594.2009.00743.x
M3 - Article
C2 - 19566736
AN - SCOPUS:67650799641
VL - 33
SP - 565
EP - 570
JO - Artificial Organs
JF - Artificial Organs
SN - 0160-564X
IS - 7
ER -