An in vivo autotransplant model of renal preservation: Cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury

Gaetano La Manna, Diletta Conte, Maria Laura Cappuccilli, Bruno Nardo, Francesca D'Addio, Lorenza Puviani, Giorgia Comai, Francesca Bianchi, Riccardo Bertelli, Nicole Lanci, Gabriele Donati, Maria Piera Scolari, Alessandro Faenza, Sergio Stefoni

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

There is increasing proof that organ preservation by machine perfusion is able to limit ischemia/reperfusion injury in kidney transplantation. This study was designed to compare the efficiency in hypothermic organ preservation by machine perfusion or cold storage in an animal model of kidney autotransplantation. Twelve pigs underwent left nephrectomy after warm ischemic time; the organs were preserved in machine perfusion (n = 6) or cold storage (n = 6) and then autotransplanted with immediate contralateral nephrectomy. The following parameters were compared between the two groups of animals: hematological and urine indexes of renal function, blood/gas analysis values, histological features, tissue adenosine-5′-triphosphate (ATP) content, perforin gene expression in kidney biopsies, and organ weight changes were compared before and after preservation. The amount of cellular ATP was significantly higher in organs preserved by machine perfusion; moreover, the study of apoptosis induction revealed an enhanced perforin expression in the kidneys, which underwent simple hypothermic preservation compared to the machine-preserved ones. Organ weight was significantly decreased after cold storage, but it remained quite stable for machine-perfused kidneys. The present model seems to suggest that organ preservation by hypothermic machine perfusion is able to better control cellular impairment in comparison with cold storage.

Original languageEnglish
Pages (from-to)565-570
Number of pages6
JournalArtificial Organs
Volume33
Issue number7
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Cold storage
Autografts
Reperfusion Injury
Organ Preservation
Perfusion
Kidney
Perforin
Adenosine
Organ Size
Blood gas analysis
Animals
Nephrectomy
Adenosine Triphosphate
Biopsy
Cell death
Warm Ischemia
Blood Gas Analysis
Gene expression
Autologous Transplantation
Kidney Transplantation

Keywords

  • Delayed graft function
  • Ischemia/reperfusion injury
  • Kidney function
  • Machine perfusion
  • Organ preservation
  • Renal transplantation

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

An in vivo autotransplant model of renal preservation : Cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury. / La Manna, Gaetano; Conte, Diletta; Cappuccilli, Maria Laura; Nardo, Bruno; D'Addio, Francesca; Puviani, Lorenza; Comai, Giorgia; Bianchi, Francesca; Bertelli, Riccardo; Lanci, Nicole; Donati, Gabriele; Scolari, Maria Piera; Faenza, Alessandro; Stefoni, Sergio.

In: Artificial Organs, Vol. 33, No. 7, 07.2009, p. 565-570.

Research output: Contribution to journalArticle

La Manna, G, Conte, D, Cappuccilli, ML, Nardo, B, D'Addio, F, Puviani, L, Comai, G, Bianchi, F, Bertelli, R, Lanci, N, Donati, G, Scolari, MP, Faenza, A & Stefoni, S 2009, 'An in vivo autotransplant model of renal preservation: Cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury', Artificial Organs, vol. 33, no. 7, pp. 565-570. https://doi.org/10.1111/j.1525-1594.2009.00743.x
La Manna, Gaetano ; Conte, Diletta ; Cappuccilli, Maria Laura ; Nardo, Bruno ; D'Addio, Francesca ; Puviani, Lorenza ; Comai, Giorgia ; Bianchi, Francesca ; Bertelli, Riccardo ; Lanci, Nicole ; Donati, Gabriele ; Scolari, Maria Piera ; Faenza, Alessandro ; Stefoni, Sergio. / An in vivo autotransplant model of renal preservation : Cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury. In: Artificial Organs. 2009 ; Vol. 33, No. 7. pp. 565-570.
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