An inductively coupled plasma mass spectrometry method for the quantification of yttrium-antibody based drugs using stable isotope tracing

Domenico Ciavardelli, Gaetano D'Anniballe, Giuseppe Nano, Franck Martin, Giorgio Federici, Paolo Sacchetta, Carmine Di Ilio, Andrea Urbani

Research output: Contribution to journalArticle

Abstract

Targeted radioimmunotherapy has been recently clinically validated and approved for the treatment of cancer by the US Food and Drug Administration. This therapeutic approach employs monoclonal antibodies directed to cancer-related, cell-surface antigens coupled to β-emitting nuclides. 90Y is one of the most useful radioisotopes in the development of antibody based radioimmunotherapy and evaluation of the pharmacokinetic profile for 90Y-radiopharmaceuticals is usually performed by radiochemical methods. In this work we have developed an alternative radioactive-free approach to evaluate pharmacokinetic profiles based on the inductively coupled plasma mass spectrometric (ICP-MS) quantification of 89Y. A highly sensitive and rapid method for the determination of yttrium in urine is described and applied to evaluate the urinary clearance of antibody-based drugs labeled with the stable isotope of yttrium, 89Y. This approach overcomes some important limitations for pre-clinical radioanalytical methods such as radiation hazards and radioactive waste disposal. Method development was performed by determining detection and quantification limits, and precision as repeatability and trueness. These performance parameters fulfilled the acceptance criteria for bioanalytical methods.

Original languageEnglish
Pages (from-to)2343-2350
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume21
Issue number14
DOIs
Publication statusPublished - 2007

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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