An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: The COMPASS-VWF study

A. Szederjesi, L. Baronciani, U. Budde, G. Castaman, A. S. Lawrie, Y. Liu, R. Montgomery, F. Peyvandi, R. Schneppenheim, A. Várkonyi, J. Patzke, I. Bodó

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Essentials: New VWF activity assays are increasingly used but information on their comparability is limited. This is an ISTH SSC-organized study (expert labs, 5 countries) to compare all available assays. VWF activity by six assays correlated well with each other. The new assays show improved characteristics - minor differences are noted. Summary: Background: Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other. Methods: The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well-characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study. Results: All tests correlated well with VWF:RCo activity (r-values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL-1. The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3-4 and 3-6 IU dL-1 range, respectively. Inter-laboratory variation was also improved for most new assays. Conclusion: All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS-VWF study will assist the VWF community in interpreting and comparing activity results.

Original languageEnglish
Pages (from-to)1604-1613
JournalJournal of Thrombosis and Haemostasis
Volume16
Issue number8
DOIs
Publication statusPublished - 2018

Fingerprint

Platelet Glycoprotein GPIb-IX Complex
von Willebrand Factor
Hemostasis
Thrombosis

Keywords

  • Clinical laboratory techniques
  • Hemorrhagic disorders
  • Hemostasis
  • Von Willebrand disease
  • Von Willebrand factor

ASJC Scopus subject areas

  • Hematology

Cite this

An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays : The COMPASS-VWF study. / Szederjesi, A.; Baronciani, L.; Budde, U.; Castaman, G.; Lawrie, A. S.; Liu, Y.; Montgomery, R.; Peyvandi, F.; Schneppenheim, R.; Várkonyi, A.; Patzke, J.; Bodó, I.

In: Journal of Thrombosis and Haemostasis, Vol. 16, No. 8, 2018, p. 1604-1613.

Research output: Contribution to journalArticle

Szederjesi, A, Baronciani, L, Budde, U, Castaman, G, Lawrie, AS, Liu, Y, Montgomery, R, Peyvandi, F, Schneppenheim, R, Várkonyi, A, Patzke, J & Bodó, I 2018, 'An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: The COMPASS-VWF study', Journal of Thrombosis and Haemostasis, vol. 16, no. 8, pp. 1604-1613. https://doi.org/10.1111/jth.14206
Szederjesi, A. ; Baronciani, L. ; Budde, U. ; Castaman, G. ; Lawrie, A. S. ; Liu, Y. ; Montgomery, R. ; Peyvandi, F. ; Schneppenheim, R. ; Várkonyi, A. ; Patzke, J. ; Bodó, I. / An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays : The COMPASS-VWF study. In: Journal of Thrombosis and Haemostasis. 2018 ; Vol. 16, No. 8. pp. 1604-1613.
@article{82f335c35b0c4fd994936c68212dbbb3,
title = "An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: The COMPASS-VWF study",
abstract = "Essentials: New VWF activity assays are increasingly used but information on their comparability is limited. This is an ISTH SSC-organized study (expert labs, 5 countries) to compare all available assays. VWF activity by six assays correlated well with each other. The new assays show improved characteristics - minor differences are noted. Summary: Background: Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other. Methods: The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well-characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study. Results: All tests correlated well with VWF:RCo activity (r-values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL-1. The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3-4 and 3-6 IU dL-1 range, respectively. Inter-laboratory variation was also improved for most new assays. Conclusion: All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS-VWF study will assist the VWF community in interpreting and comparing activity results.",
keywords = "Clinical laboratory techniques, Hemorrhagic disorders, Hemostasis, Von Willebrand disease, Von Willebrand factor",
author = "A. Szederjesi and L. Baronciani and U. Budde and G. Castaman and Lawrie, {A. S.} and Y. Liu and R. Montgomery and F. Peyvandi and R. Schneppenheim and A. V{\'a}rkonyi and J. Patzke and I. Bod{\'o}",
year = "2018",
doi = "10.1111/jth.14206",
language = "English",
volume = "16",
pages = "1604--1613",
journal = "Journal of Thrombosis and Haemostasis",
issn = "1538-7933",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays

T2 - The COMPASS-VWF study

AU - Szederjesi, A.

AU - Baronciani, L.

AU - Budde, U.

AU - Castaman, G.

AU - Lawrie, A. S.

AU - Liu, Y.

AU - Montgomery, R.

AU - Peyvandi, F.

AU - Schneppenheim, R.

AU - Várkonyi, A.

AU - Patzke, J.

AU - Bodó, I.

PY - 2018

Y1 - 2018

N2 - Essentials: New VWF activity assays are increasingly used but information on their comparability is limited. This is an ISTH SSC-organized study (expert labs, 5 countries) to compare all available assays. VWF activity by six assays correlated well with each other. The new assays show improved characteristics - minor differences are noted. Summary: Background: Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other. Methods: The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well-characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study. Results: All tests correlated well with VWF:RCo activity (r-values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL-1. The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3-4 and 3-6 IU dL-1 range, respectively. Inter-laboratory variation was also improved for most new assays. Conclusion: All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS-VWF study will assist the VWF community in interpreting and comparing activity results.

AB - Essentials: New VWF activity assays are increasingly used but information on their comparability is limited. This is an ISTH SSC-organized study (expert labs, 5 countries) to compare all available assays. VWF activity by six assays correlated well with each other. The new assays show improved characteristics - minor differences are noted. Summary: Background: Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other. Methods: The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well-characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study. Results: All tests correlated well with VWF:RCo activity (r-values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL-1. The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3-4 and 3-6 IU dL-1 range, respectively. Inter-laboratory variation was also improved for most new assays. Conclusion: All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS-VWF study will assist the VWF community in interpreting and comparing activity results.

KW - Clinical laboratory techniques

KW - Hemorrhagic disorders

KW - Hemostasis

KW - Von Willebrand disease

KW - Von Willebrand factor

UR - http://www.scopus.com/inward/record.url?scp=85050625264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050625264&partnerID=8YFLogxK

U2 - 10.1111/jth.14206

DO - 10.1111/jth.14206

M3 - Article

AN - SCOPUS:85050625264

VL - 16

SP - 1604

EP - 1613

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

SN - 1538-7933

IS - 8

ER -