An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues

Chang Gong Liu, George Adrian Calin, Brian Meloon, Nir Gamliel, Cinzia Sevignani, Manuela Ferracin, Calin Dan Dumitru, Masayoshi Shimizu, Simona Zupo, Mariella Dono, Hansjuerg Alder, Florencia Bullrich, Massimo Negrini, Carlo M. Croce

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.

Original languageEnglish
Pages (from-to)9740-9744
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number26
DOIs
Publication statusPublished - Jun 29 2004

ASJC Scopus subject areas

  • Genetics
  • General

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