An on-line semi-automated solid-phase extraction procedure for high-performance liquid chromatographic determination of lonidamine in serum

Clementina Bottalico, Giuseppina Micelli, Antonio Guerrieri, Francesco Palmisano, Vito Lorusso, Mario De Lena

Research output: Contribution to journalArticlepeer-review

Abstract

A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 μg ml-1 level was (92.8 ± 3.4)%. The limit of quantitation for a 100 μl sample size was 50 ng ml-1. The within-day (n = 5) and between-day (n = 5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 μg ml-1 level were 2.7% and 4.5%, respectively.

Original languageEnglish
Pages (from-to)1349-1353
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume13
Issue number11
DOIs
Publication statusPublished - 1995

Keywords

  • Lonidamine
  • Reversed-phase liquid chromatography
  • Solid-phase extraction
  • UV detection

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Drug Discovery
  • Pharmaceutical Science

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