An original application of plasma expanders: Heart-lung preservation

L. Spaggiari, M. Rusca, P. Carbognani, R. Alfieri, S. Urbani, P. G. Petronini, L. Cattelani, A. F. Borghetti, P. Bobbio

Research output: Contribution to journalArticlepeer-review

Abstract

The lack of an ideal heart-lung preservation solution is one of the principal factor that limits the wide spread of transplantation. The aim of this work was to investigate the efficacy of Haemaccel (HM) on isolated human pulmonary artery endothelial cells comparing its effects with those of University of Wisconsin (UWS). Subcultures of HPAEC were inoculated at the density of 5,000 cells per cm2 in 9 cm2 well-plates. Cells were incubated with HM and UWS for 6 hrs at 10°C. Cellular viability was analysed by the total protein content (cytotoxicity index) and by the rate of protein synthesis (metabolic index). The results showed that HM and UWS did no show a significant differences in the toxicity when compared with the control; on the contrary, HM seems to determine a less inhibitory effect on cellular metabolism permitting a more rapid cellular metabolic recovery than UWS. Thus, HM appears to be more suitable for the preservation of isolated HPAEC than UWS.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalJournal of Cardiovascular Surgery
Volume37
Issue number6 SUPPL. 1
Publication statusPublished - Dec 1996

Keywords

  • Heart-lung preservation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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