An SEM analysis of the interaction between lymphokine-activated killer cells and tumor targets

The interaction between lymphokine-activated killer (LAK) cells and two types of target cells with different susceptibility to natural killer (NK) activity was investigated by scanning electron microscopy (SEM). In NK-susceptible tumor cells (K562) a different mode of conjugation with the effector was obeserved as compared with NK-resistant targets (YHP-1-0). In LAK-K562 pairs, the contact region was characterized by the presence of long microvilli, blebs and ruffled membranes forming an intertwined and interdigitated binding site. Conversely, when LAK cells were conjugated with THP-1-0-cells, the surfaced structures of the target cell dit not undergo significant modification and the interacting cells did not appear to establish close contact. In addition, cell lysis of the sensitive target was often characterized by plasma membrane blebbing, leading to cell death. In contrast, in the low percentage of resistant targets which underwent lysis after conjugation, cell death always occurred without formation of bulb- or bleb-like structures.